Detailed information of TA system TA021924

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): VapXD/-
• Location: 5528760..5529238
• Replicon: chromosome
• Accession: NC_008786
• Organism: Verminephrobacter eiseniae EF01-2

Toxin (Protein)

• Gene name: VapD
• Uniprot ID: A1WSS3
• Locus tag: VEIS_RS24230
• Protein ID: WP_011812658.1
• Coordinates: 5528960..5529238 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: VapX
• Uniprot ID: -
• Locus tag: VEIS_RS24225
• Protein ID: WP_041951271.1
• Coordinates: 5528760..5528960 (+)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VEIS_RS24215	5525513..5527939	+	2427	WP_011812655.1	ABC transporter ATP-binding protein/permease	-
VEIS_RS24220	5527991..5528485	+	495	WP_041950329.1	DUF1854 domain-containing protein	-
VEIS_RS24225	5528760..5528960	+	201	WP_041951271.1	DUF5397 domain-containing protein	Antitoxin
VEIS_RS24230	5528960..5529238	+	279	WP_011812658.1	virulence factor	Toxin
VEIS_RS24235	5529409..5529846	+	438	WP_041950330.1	hypothetical protein	-
VEIS_RS29445	5530059..5530661	+	603	WP_198137936.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Virulence-associated protein D / CRISPR associated protein Cas2 (1-87)

Antitoxin

• Protein of unknown function DUF5397 (4-66)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10501.05 Da
• Isoelectric Point: 8.4848

>T21924 WP_011812658.1 NC_008786:5528960-5529238 [Verminephrobacter eiseniae EF01-2]
MYALAFDLVVADTAKHHPKGVTQAYTEIGAVLGEYGFRRVQGSLYVTDDENMANLFLAIQALRTRMWFPKSVRDIRAFRI
EQWSDFTAVVKS

Nucleotide

• Length: 279 bp

>T21924 NC_008786:5528960-5529238 [Verminephrobacter eiseniae EF01-2]
ATGTACGCGCTAGCTTTCGACCTGGTGGTGGCCGACACTGCCAAGCATCACCCCAAGGGCGTGACACAGGCGTATACCGA
GATTGGCGCGGTGCTTGGCGAGTATGGCTTTCGCCGCGTACAGGGTAGCCTGTACGTCACCGATGACGAGAACATGGCGA
ACCTGTTCCTTGCCATCCAGGCGCTGAGAACCCGGATGTGGTTCCCAAAGTCCGTGCGCGATATCCGCGCTTTCCGTATC
GAGCAGTGGTCTGACTTCACTGCCGTGGTGAAGTCGTAA

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7383.40 Da
• Isoelectric Point: 4.6436

>AT21924 WP_041951271.1 NC_008786:5528760-5528960 [Verminephrobacter eiseniae EF01-2]
MQTAITPPAVPVGEFKSFGPLGPKYEVGRAIRQLGDGDWMIEITMIETGEKAEYRWSHLIDDPQAH

Nucleotide

• Length: 201 bp

>AT21924 NC_008786:5528760-5528960 [Verminephrobacter eiseniae EF01-2]
ATGCAGACAGCCATCACACCGCCCGCCGTTCCGGTCGGCGAATTCAAGAGCTTTGGGCCGTTGGGCCCGAAATACGAAGT
GGGTCGAGCCATTCGCCAGTTGGGCGATGGGGACTGGATGATCGAGATCACGATGATCGAAACGGGCGAGAAAGCGGAAT
ACCGTTGGTCGCATCTGATCGATGACCCGCAGGCTCACTGA

Structures

Toxin

• AlphaFold DB: A1WSS3