219231

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 2762..3350 Replicon plasmid pHKU6a
Accession NZ_CP084725
Organism Acinetobacter baumannii strain HKU6

Toxin (Protein)


Gene name brnT Uniprot ID A0A3G6Z3M6
Locus tag LHJ64_RS19355 Protein ID WP_000438826.1
Coordinates 2762..3049 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID N9M5I3
Locus tag LHJ64_RS19360 Protein ID WP_001983304.1
Coordinates 3036..3350 (+) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LHJ64_RS19335 (LHJ64_19335) 1..951 + 951 WP_001205343.1 replication initiation protein RepM -
LHJ64_RS19340 (LHJ64_19340) 944..1519 + 576 WP_001096616.1 plasmid replication DNA-binding protein -
LHJ64_RS19345 (LHJ64_19345) 1539..1682 + 144 WP_001125246.1 hypothetical protein -
LHJ64_RS19350 (LHJ64_19350) 1871..2389 - 519 WP_000447193.1 tetratricopeptide repeat protein -
LHJ64_RS19355 (LHJ64_19355) 2762..3049 + 288 WP_000438826.1 BrnT family toxin Toxin
LHJ64_RS19360 (LHJ64_19360) 3036..3350 + 315 WP_001983304.1 BrnA antitoxin family protein Antitoxin
LHJ64_RS19365 (LHJ64_19365) 3478..5889 + 2412 WP_000932953.1 TonB-dependent receptor ZnuD2 -
LHJ64_RS19370 (LHJ64_19370) 6194..6655 + 462 WP_000761346.1 DIP1984 family protein -
LHJ64_RS19375 (LHJ64_19375) 6978..7151 - 174 WP_001282484.1 hypothetical protein -
LHJ64_RS19380 (LHJ64_19380) 7151..7522 - 372 WP_000504218.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..8731 8731


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(41-96)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11303.72 Da        Isoelectric Point: 6.0889

>T219231 WP_000438826.1 NZ_CP084725:2762-3049 [Acinetobacter baumannii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Download         Length: 288 bp

>T219231 NZ_CP116217:c5318-5013 [Klebsiella michiganensis]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGGCGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTCTCCCGTGAGCTCTATCCGGTGG
TGCACATCGGGGACGAGAGCTGGCGCCTGATGACCACGGACATGGCCAGCGTCCCGGCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCCGCCACCGGGAAAATGACATCAAAAACGCAATTAACCTGATGTTCTGGGGGATCTGA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 12208.84 Da        Isoelectric Point: 7.2026

>AT219231 WP_001983304.1 NZ_CP084725:3036-3350 [Acinetobacter baumannii]
MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Download         Length: 315 bp

>AT219231 NZ_CP116217:c5538-5320 [Klebsiella michiganensis]
ATGAAGCAGCGCATCACCGTCACCATCGACAGCGACAGCTATCAGTTACTGAAATCCGCGGACGTCAATATCTCCGGCCT
GGTGAACAGGGCCATGCAGAATGAAGCCCGACGTCTGCGTGCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCTG
AGGTTGCCCGGTTTATTGAAATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G6Z3M6

Antitoxin

Source ID Structure
AlphaFold DB N9M5I3

References