Detailed information of TA system TA219223

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 3421349..3421865
• Replicon: chromosome
• Accession: NZ_CP084714
• Organism: Pseudomonas asiatica strain P1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: F8G1E7
• Locus tag: LG197_RS15690
• Protein ID: WP_003259987.1
• Coordinates: 3421584..3421865 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: LG197_RS15685
• Protein ID: WP_096010237.1
• Coordinates: 3421349..3421594 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LG197_RS15665 (LG197_15660)	3416776..3417318	-	543	WP_038409286.1	WYL domain-containing protein	-
LG197_RS15670 (LG197_15665)	3417426..3418412	-	987	WP_196184776.1	zinc-binding dehydrogenase	-
LG197_RS15675 (LG197_15670)	3418775..3419968	+	1194	WP_196184775.1	MFS transporter	-
LG197_RS15680 (LG197_15675)	3419999..3421105	+	1107	WP_196184774.1	alkene reductase	-
LG197_RS15685 (LG197_15680)	3421349..3421594	+	246	WP_096010237.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LG197_RS15690 (LG197_15685)	3421584..3421865	+	282	WP_003259987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LG197_RS15695 (LG197_15690)	3421904..3422299	-	396	WP_023662326.1	hypothetical protein	-
LG197_RS15700 (LG197_15695)	3422388..3423296	-	909	WP_013972045.1	LysR family transcriptional regulator	-
LG197_RS15705 (LG197_15700)	3423588..3424892	+	1305	WP_013972046.1	MFS transporter	-
LG197_RS15710 (LG197_15705)	3424923..3426164	+	1242	WP_196184772.1	Zn-dependent hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (10-76)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10909.78 Da
• Isoelectric Point: 10.7594

>T219223 WP_003259987.1 NZ_CP084714:3421584-3421865 [Pseudomonas asiatica]
MTYKLEFLPSALKEWGKLGHTVREQIKKKLAERLQSPKVQADALRDLPNHYKIKLKASGYRLVYRVEDERIVVVVVSVGK
RERSEVYRSAQKR

Nucleotide

• Length: 282 bp

>T219223 NZ_CP116214:4525725-4525943 [Klebsiella michiganensis]
ATGTCTGATAAAACATTAACCAAAATAGACTATTTGATGCGTTTACGACGCTGTCAGACAATTGATACGCTGGAGCGCGT
AATTGAAAAAAATAAATATGAACTTTCCGATAATGAACTGGCGGTATTTTACTCTGCGGCCGATCATCGTCTTGCAGAGC
TGACGATGAATAAACTGTATGACAAGATCCCCCCTTCAGTCTGGAAATTTATCCGCTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8749.18 Da
• Isoelectric Point: 4.3611

>AT219223 WP_096010237.1 NZ_CP084714:3421349-3421594 [Pseudomonas asiatica]
MQNIFADVAVSVSELKKNPSAVMTGAAGMPVAVLNHNRVMGYMVPAELYEAMMERLEDLELGELAKSRLGEKGVPVSLDD
L

Nucleotide

• Length: 246 bp

>AT219223 NZ_CP116214:4525324-4525698 [Klebsiella michiganensis]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCGCAGCTTAAATTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTGGAAGAGAGCAACCACGGCTGGGTTAATGATCCAACCTCGGCTGTCAATCTTCAACTTAATGAATTGATCGAGC
ACATTGCCGCGTTTGCACTTAATTATAAAATTAAGTACGCAGAGGATAATAAGCTGATTACCCAGATTGACGAATATCTG
GATGACACTTTCGTCTTGTTCAGCAACTACGGCATTAACAGCGCAGATCTGCAGAAATGGCGTAAATCTGGCAATCGGTT
ATTCCGCTGCTTTGTCAACGCCAGTCGGGAAAACCCGGCCAGTCTTTCTTGTTAG

Structures

Toxin

• AlphaFold DB: F8G1E7