Detailed information of TA system TA219221

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 5365196..5365859
• Replicon: chromosome
• Accession: NZ_CP084713
• Organism: Mycolicibacterium phocaicum strain RL-HY01

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LHJ73_RS26110
• Protein ID: WP_212807252.1
• Coordinates: 5365431..5365859 (+)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LHJ73_RS26105
• Protein ID: WP_212814558.1
• Coordinates: 5365196..5365444 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LHJ73_RS26085 (LHJ73_26085)	5360977..5361678	+	702	WP_052543539.1	plasmid pRiA4b ORF-3 family protein	-
LHJ73_RS26090 (LHJ73_26090)	5361686..5363008	-	1323	WP_052545499.1	ISL3 family transposase	-
LHJ73_RS26095 (LHJ73_26095)	5363122..5363337	-	216	WP_212807250.1	hypothetical protein	-
LHJ73_RS26100 (LHJ73_26100)	5363697..5365088	-	1392	WP_226519829.1	MFS transporter	-
LHJ73_RS26105 (LHJ73_26105)	5365196..5365444	+	249	WP_212814558.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LHJ73_RS26110 (LHJ73_26110)	5365431..5365859	+	429	WP_212807252.1	type II toxin-antitoxin system VapC family toxin	Toxin
LHJ73_RS26115 (LHJ73_26115)	5365862..5366431	-	570	WP_226519830.1	flavin reductase family protein	-
LHJ73_RS26120 (LHJ73_26120)	5366443..5367345	-	903	WP_226519831.1	VOC family protein	-
LHJ73_RS26125 (LHJ73_26125)	5367358..5368224	-	867	WP_226519832.1	alpha/beta fold hydrolase	-
LHJ73_RS26130 (LHJ73_26130)	5368224..5369414	-	1191	WP_053855346.1	flavin-dependent monooxygenase	-
LHJ73_RS26135 (LHJ73_26135)	5369567..5370640	+	1074	WP_226519833.1	ferredoxin--NADP reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	5361686..5362894	1208

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-141)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-41)

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15687.93 Da
• Isoelectric Point: 7.3846

>T219221 WP_212807252.1 NZ_CP084713:5365431-5365859 [Mycolicibacterium phocaicum]
MLLVDVNIWVYAIRSDSAQHGTYREWVEEALTGSEPVGVSELVLSGVMRLITNHRIFSQPSSTAEALDACRAIRSAPSAI
SLRPGPRHWSIFEKLCTTVGAKANVVPDAYHAALAIEHGATWVTTDRAFARFPGLKWRLPFE

Nucleotide

• Length: 429 bp

>T219221 NZ_CP116214:2055551-2055883 [Klebsiella michiganensis]
ATGGACAGAGGGGAAATCTGGCTCGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGCGGAAAACGTCCGGTGCTTAT
CGTATCGAAGGCATCGTTTAACAAGCTGACCCGGTTACCCGTTGTTGTTCCCATCACCAGCGGTGGAAATTTTGCTCGCA
CGGCAGGTTTCACCGTTTCACTGGAAGGGGCAGGGACAAAAACAACGGGCGTTATTCGCTGCGACCAGCCCAGAACCATT
GATATGGCTGCCCGGAACGGCAAGCGGCTGGAAAGCATACCAGACGCTGTGGTAAATGAAGTGCTTGCCAGGCTGGATGC
AATTTTAAGTTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8613.67 Da
• Isoelectric Point: 5.0069

>AT219221 WP_212814558.1 NZ_CP084713:5365196-5365444 [Mycolicibacterium phocaicum]
MRTTVNIDDHLLEEAKVLAARTSRSLGSVVEDGLRALLHRNNPGTSVGTFVLPTHGIGGLAPGVDLEDKDALAELTGDNA
PR

Nucleotide

• Length: 249 bp

>AT219221 NZ_CP116214:2055293-2055550 [Klebsiella michiganensis]
ATGTATACCACTCGCCTGAAAAAGGTCGGCGGATCCATCATGTTGGCGGTTCCTCCCGCCGTGCTCAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTCGGCATGACCATTAATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCTCATTATTCTC
TTGAGGAGCTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCTCCTGCGGTG
GGCAAGGAGATCCTGTAG