Detailed information of TA system TA219219

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-ParD
• Location: 3004245..3005218
• Replicon: chromosome
• Accession: NZ_CP084713
• Organism: Mycolicibacterium phocaicum strain RL-HY01

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: -
• Locus tag: LHJ73_RS14375
• Protein ID: WP_226522015.1
• Coordinates: 3004643..3005218 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: -
• Locus tag: LHJ73_RS14370
• Protein ID: WP_212811429.1
• Coordinates: 3004245..3004646 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LHJ73_RS14350 (LHJ73_14350)	3000020..3001339	-	1320	WP_016894482.1	IS256 family transposase	-
LHJ73_RS14355 (LHJ73_14355)	3001500..3001988	+	489	WP_226522012.1	hypothetical protein	-
LHJ73_RS14360 (LHJ73_14360)	3003033..3003728	+	696	WP_226522013.1	hypothetical protein	-
LHJ73_RS14365 (LHJ73_14365)	3003990..3004136	-	147	WP_226522014.1	hypothetical protein	-
LHJ73_RS14370 (LHJ73_14370)	3004245..3004646	+	402	WP_212811429.1	hypothetical protein	Antitoxin
LHJ73_RS14375 (LHJ73_14375)	3004643..3005218	+	576	WP_226522015.1	zeta toxin family protein	Toxin
LHJ73_RS14380 (LHJ73_14380)	3005728..3006279	+	552	WP_226522016.1	YaeQ family protein	-
LHJ73_RS14385 (LHJ73_14385)	3006393..3006944	+	552	WP_226522017.1	DapH/DapD/GlmU-related protein	-
LHJ73_RS14390 (LHJ73_14390)	3007269..3007415	-	147	WP_226522018.1	hypothetical protein	-
LHJ73_RS14395 (LHJ73_14395)	3007702..3008013	+	312	WP_226522019.1	NIPSNAP family protein	-
LHJ73_RS14400 (LHJ73_14400)	3008049..3008627	+	579	WP_226522020.1	TetR/AcrR family transcriptional regulator	-
LHJ73_RS14405 (LHJ73_14405)	3008707..3009498	+	792	WP_226522021.1	SDR family NAD(P)-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2984402..3011290	26888
	-	flank	IS/Tn	-	-	3000020..3001339	1319

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (4-142)

Antitoxin

• ParD-like antitoxin of type II bacterial toxin-antitoxin system (6-106)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 20966.93 Da
• Isoelectric Point: 9.3920

>T219219 WP_226522015.1 NZ_CP084713:3004643-3005218 [Mycolicibacterium phocaicum]
VKRLDLVVGSNGAGKSTFVELTLAPLLPSSVFVNADEIAKQRWPTDALGHAYEAARIAAATREALIEQGRSFIAETVFSH
PSKLELIDAARHAGYVVVLHIVLIPENLAVHRVRYRVAAGGHDVPEEKIRERYRRLWTLVAAAIVRVDSAAVYDNSSASG
PQIVAQMSGGLSVDTPRWPSWTPEQLRSRWP

Nucleotide

• Length: 576 bp

>T219219 NZ_CP116214:846674-847084 [Klebsiella michiganensis]
GTGGTCCTGTGGCAATCTGATTTACGCATCTCGTGGCGAGCGCAGTGGTTTTCGCTGCTGATGCACGGCGTGGTGGCGGC
GCTTGTGCTGCTGATGCCCTGGCCGCTGAGCTATACCCCGCTGTGGCTGATACTGCTGTCGCTGGTGGTGTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCAGGGAGAAATCAAGCTGCTGATCGACTCCCGTCTGCGCTGGCAAAAGGCG
GAGTGGGATATCATCGGCACCCCGTGGGTGATTAACAGCGGAATGCTGCTGCGCCTGCGGAATACGGAAAACCAACGAAC
CCAGCATTTGTGGGTCGCGGCGGATAGCATGGACGCGGGAGAATGGCGAGACCTGCGTCGGCTGGTGTTGCTAAAACCGA
CGCAAGAGTAG

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14192.69 Da
• Isoelectric Point: 5.2297

>AT219219 WP_212811429.1 NZ_CP084713:3004245-3004646 [Mycolicibacterium phocaicum]
MSEVADRVTRVSADLFEAAAAEGARQSRSAKQQLDHWARVGRAVSTQHTASRRRVEAALAGQIDIAVLNTEEGVVFNAEV
SAAIEESLARTDYGAVLASRGITTVALDEDGQIVEHRPDGTESVVGAVHSLRR

Nucleotide

• Length: 402 bp

>AT219219 NZ_CP116214:846427-846693 [Klebsiella michiganensis]
ATGGACATTAACAATAAAGCCCGCATCCACTGGGCATGCCGTCGAGGTATGCGTGAACTCGATATCTCCATCATGCCGTT
TTTCGAGCATGAGTACGACACGCTCAGCGATGGCGATAAAGCGTTATTCATCCGCCTGCTGGAAAACGACGATCCGGATC
TGTTCAACTGGTTGATGAATCATGGCGAGCCGGCTGATGCAGAACTGCAGCGGATGGTAAAATTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA