Detailed information of TA system TA219218

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2466647..2467299
• Replicon: chromosome
• Accession: NZ_CP084713
• Organism: Mycolicibacterium phocaicum strain RL-HY01

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LHJ73_RS11780
• Protein ID: WP_226521712.1
• Coordinates: 2466904..2467299 (+)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A7I7ZJC5
• Locus tag: LHJ73_RS11775
• Protein ID: WP_064859434.1
• Coordinates: 2466647..2466907 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LHJ73_RS11765 (LHJ73_11765)	2463237..2465270	-	2034	WP_060999715.1	toprim domain-containing protein	-
LHJ73_RS11770 (LHJ73_11770)	2465411..2466607	+	1197	WP_226521711.1	crosslink repair DNA glycosylase YcaQ family protein	-
LHJ73_RS11775 (LHJ73_11775)	2466647..2466907	+	261	WP_064859434.1	CopG family transcriptional regulator	Antitoxin
LHJ73_RS11780 (LHJ73_11780)	2466904..2467299	+	396	WP_226521712.1	type II toxin-antitoxin system VapC family toxin	Toxin
LHJ73_RS11785 (LHJ73_11785)	2467286..2467945	-	660	WP_226521713.1	DUF4304 domain-containing protein	-
LHJ73_RS11790 (LHJ73_11790)	2468047..2468721	-	675	WP_226521714.1	hypothetical protein	-
LHJ73_RS11795 (LHJ73_11795)	2468878..2469270	-	393	WP_226521715.1	Lsr2 family protein	-
LHJ73_RS11800 (LHJ73_11800)	2469451..2469849	-	399	WP_226521716.1	VOC family protein	-
LHJ73_RS11805 (LHJ73_11805)	2469872..2471278	-	1407	WP_226521717.1	APC family permease	-
LHJ73_RS11810 (LHJ73_11810)	2471308..2472225	-	918	WP_268952913.1	proline iminopeptidase-family hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-110)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-39)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14461.83 Da
• Isoelectric Point: 8.8084

>T219218 WP_226521712.1 NZ_CP084713:2466904-2467299 [Mycolicibacterium phocaicum]
MMLVDSDILIAHLRGIEVARDWLVSARRAGPLSISVVTVTELVGGMRSPERRAVWRLLDSMTVKPINELVARRAGDFMRE
HRRSNSGIGLADYLIAATAEINGLDLATLNIRHFPMFDGLEAPFALPASPR

Nucleotide

• Length: 396 bp

>T219218 NZ_CP116214:691398-691739 [Klebsiella michiganensis]
ATGAAAACTCTACCTGCTACAATTTCGCGGGCGGCGAAGCCCTGTCTGTCGCCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGCCTGCTGGAACAGCACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATTG
ATGCCGGGATTACCCTGGCCGATGCAGTCAACTTCCTGGTGGATAAGTACGAACTGGTTCGTATCGATCGCAGGGGGTTT
AACTGGCAGGAGCAATCCCCTTATCTTCGGGCGGTAGATATCCTGCGAGCACGACAAGCAACTGGCTTGTTGCGACGGAG
CCGTAATAACGCTGTATCGTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9615.70 Da
• Isoelectric Point: 4.1844

>AT219218 WP_064859434.1 NZ_CP084713:2466647-2466907 [Mycolicibacterium phocaicum]
MKRTNIYLDEEQAALLDQLAAQEGLSRAELIRRMLDKALTGGGDSTLEADVAVLNDSFGVACDIAWPVRETGDREEHLEQ
VWNRTQ

Nucleotide

• Length: 261 bp

>AT219218 NZ_CP116214:691036-691377 [Klebsiella michiganensis]
ATGAAATCTACTGACTCAGAGAATGATTCATTTCTCAAATGGGGTCTGAATCGTAATGAGACACCTTGCTTTGGCGCTCG
TCTGGTACAGGAGGGAAACCGACTTCACTATCTGGCTAACCGGGCCAGCATTACCGGTGAATTTATTAACACAGAATATT
TGAAGTTGGATGAAATATTTCCGCATTTTATCAGCCAGATGGAATCGATGCTGACCACTGGTGAAATGAATCCTCGCCAT
GCCCACTGCGTCACTCTATACCACAACGGTTTCACCTGCGAAGCCGATACACTTGGCAGTTGCGGCTACGTATACATCTC
CGTTTACCCTACTCAACGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A7I7ZJC5