Detailed information of TA system TA219214

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1419169..1420009
• Replicon: chromosome
• Accession: NZ_CP084713
• Organism: Mycolicibacterium phocaicum strain RL-HY01

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: LHJ73_RS06850
• Protein ID: WP_226521127.1
• Coordinates: 1419169..1419696 (-)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: LHJ73_RS06855
• Protein ID: WP_226521128.1
• Coordinates: 1419707..1420009 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LHJ73_RS06830 (LHJ73_06830)	1415146..1417560	+	2415	WP_226521123.1	ATP-binding protein	-
LHJ73_RS06835 (LHJ73_06835)	1417557..1417895	+	339	WP_226521124.1	hypothetical protein	-
LHJ73_RS06840 (LHJ73_06840)	1417925..1418284	-	360	WP_226521125.1	hypothetical protein	-
LHJ73_RS06845 (LHJ73_06845)	1418543..1418956	-	414	WP_226521126.1	helix-turn-helix domain-containing protein	-
LHJ73_RS06850 (LHJ73_06850)	1419169..1419696	-	528	WP_226521127.1	GNAT family N-acetyltransferase	Toxin
LHJ73_RS06855 (LHJ73_06855)	1419707..1420009	-	303	WP_226521128.1	DUF1778 domain-containing protein	Antitoxin
LHJ73_RS06860 (LHJ73_06860)	1420159..1420812	+	654	WP_226521129.1	DUF2510 domain-containing protein	-
LHJ73_RS06865 (LHJ73_06865)	1421043..1421738	+	696	WP_226521130.1	hypothetical protein	-
LHJ73_RS06870 (LHJ73_06870)	1422036..1422845	+	810	WP_226521131.1	hypothetical protein	-
LHJ73_RS06875 (LHJ73_06875)	1422880..1424082	-	1203	WP_226521132.1	Fic family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1365252..1439182	73930

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (30-141)

Antitoxin

• Vibrio phage ICP1, Orf50 (16-92)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19502.18 Da
• Isoelectric Point: 8.1655

>T219214 WP_226521127.1 NZ_CP084713:c1419696-1419169 [Mycolicibacterium phocaicum]
MTSEHKPAPPEKLAKHHDRTHFSSGATELDDWLRKYSWQNQRANNATTYVTAHQERVVGYYAITVAGYERDSAPPAIAKT
APATVPCYLLARLAVDQQWHGRGLGWGLLRDAMLRVLMLSQSVAAPALLVHARDDAAREFYLHHAEFVQSPVDPLHLLIP
VKAIAAAVLDDGRHR

Nucleotide

• Length: 528 bp

>T219214 NZ_CP116204:249732-250082 [Bacillus paranthracis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11475.09 Da
• Isoelectric Point: 7.0154

>AT219214 WP_226521128.1 NZ_CP084713:c1420009-1419707 [Mycolicibacterium phocaicum]
MPRIDIATAKTARDQRLNVRVSARQQQLIRQAAEASDTTVTDFILASVLDSAERVLADRRWFVADDHQWAEFQALLDAPL
QPMPKLRRLLMRENPFATTE

Nucleotide

• Length: 303 bp

>AT219214 NZ_CP116204:249440-249727 [Bacillus paranthracis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA