Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 507503..508139 Replicon chromosome
Accession NZ_CP084711
Organism Bacillus pumilus strain AR03

Toxin (Protein)


Gene name mazF Uniprot ID K2MHT4
Locus tag LG401_RS02480 Protein ID WP_003214169.1
Coordinates 507789..508139 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A267X601
Locus tag LG401_RS02475 Protein ID WP_012008995.1
Coordinates 507503..507784 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LG401_RS02455 (LG401_02455) 503649..504254 - 606 WP_060595589.1 rhomboid family intramembrane serine protease -
LG401_RS02460 (LG401_02460) 504349..504714 + 366 WP_034663960.1 holo-ACP synthase -
LG401_RS02465 (LG401_02465) 504875..505891 + 1017 WP_106037124.1 outer membrane lipoprotein carrier protein LolA -
LG401_RS02470 (LG401_02470) 506028..507209 + 1182 WP_226509363.1 alanine racemase -
LG401_RS02475 (LG401_02475) 507503..507784 + 282 WP_012008995.1 hypothetical protein Antitoxin
LG401_RS02480 (LG401_02480) 507789..508139 + 351 WP_003214169.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
LG401_RS02485 (LG401_02485) 508257..509132 + 876 WP_045210228.1 RsbT co-antagonist protein RsbRA -
LG401_RS02490 (LG401_02490) 509092..509460 + 369 WP_003214235.1 RsbT antagonist protein RsbS -
LG401_RS02495 (LG401_02495) 509463..509864 + 402 WP_060595592.1 anti-sigma regulatory factor -
LG401_RS02500 (LG401_02500) 509875..510882 + 1008 WP_060595593.1 PP2C family protein-serine/threonine phosphatase -
LG401_RS02505 (LG401_02505) 510942..511271 + 330 WP_060595594.1 anti-sigma factor antagonist -
LG401_RS02510 (LG401_02510) 511268..511756 + 489 WP_060595595.1 anti-sigma B factor RsbW -
LG401_RS02515 (LG401_02515) 511722..512510 + 789 WP_034663946.1 RNA polymerase sigma factor SigB -
LG401_RS02520 (LG401_02520) 512510..513109 + 600 WP_034663944.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12976.99 Da        Isoelectric Point: 5.1663

>T219210 WP_003214169.1 NZ_CP084711:507789-508139 [Bacillus pumilus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEINAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEALQVSLALIDF

Download         Length: 351 bp

>T219210 NZ_CP116199:21592-22008 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10894.27 Da        Isoelectric Point: 5.2726

>AT219210 WP_012008995.1 NZ_CP084711:507503-507784 [Bacillus pumilus]
MSESSATNEMKIRLPETLMSEIERVAEQDKVTRNELIYRATKTYIRERNIRQNRESMRRGYMEMAKINLNISSEAQYAEF
EAENTVERLVSGG

Download         Length: 282 bp

>AT219210 NZ_CP116199:21365-21595 [Escherichia coli]
ATGAGAACCGTATCGATATTCAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K2MHT4

Antitoxin

Source ID Structure
AlphaFold DB A0A267X601

References