Detailed information of TA system TA219202

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 90945..91615
• Replicon: plasmid p1
• Accession: NZ_CP084706
• Organism: Klebsiella pneumoniae strain KP1064WHY

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: K8U55_RS27815
• Protein ID: WP_004213072.1
• Coordinates: 90945..91388 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: K8U55_RS27820
• Protein ID: WP_004213073.1
• Coordinates: 91385..91615 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8U55_RS27780 (K8U55_27775)	86357..86632	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
K8U55_RS27785 (K8U55_27780)	86695..87186	+	492	WP_011251327.1	DM13 domain-containing protein	-
K8U55_RS27790 (K8U55_27785)	87235..88155	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
K8U55_RS27795 (K8U55_27790)	88246..88649	+	404	Protein_105	GAF domain-containing protein	-
K8U55_RS27800 (K8U55_27795)	89166..89801	-	636	Protein_106	mucoid phenotype regulator RmpA2	-
K8U55_RS27805 (K8U55_27800)	90218..90522	+	305	Protein_107	transposase	-
K8U55_RS27810 (K8U55_27805)	90545..90796	-	252	WP_186987481.1	hypothetical protein	-
K8U55_RS27815 (K8U55_27810)	90945..91388	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
K8U55_RS27820 (K8U55_27815)	91385..91615	-	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
K8U55_RS27825 (K8U55_27820)	92223..93356	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
K8U55_RS27830 (K8U55_27825)	93372..93665	+	294	WP_004213076.1	hypothetical protein	-
K8U55_RS27835 (K8U55_27830)	93655..93861	-	207	WP_004213077.1	hypothetical protein	-
K8U55_RS27840 (K8U55_27835)	94213..94503	+	291	WP_004213078.1	hypothetical protein	-
K8U55_RS27845 (K8U55_27840)	94493..95392	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA / iroN / iucA / iucB / iucC / iucC / iucD / iutA / rmpA2	1..201125	201125

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T219202 WP_004213072.1 NZ_CP084706:c91388-90945 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T219202 NZ_CP116197:3480246-3480464 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT219202 WP_004213073.1 NZ_CP084706:c91615-91385 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT219202 NZ_CP116197:3479846-3480220 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620