Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 49798..50708 Replicon plasmid p1
Accession NZ_CP084706
Organism Klebsiella pneumoniae strain KP1064WHY

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag K8U55_RS27565 Protein ID WP_004026354.1
Coordinates 49798..50268 (-) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag K8U55_RS27570 Protein ID WP_004026357.1
Coordinates 50265..50708 (-) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8U55_RS27525 (K8U55_27520) 45114..45428 + 315 WP_011251273.1 hypothetical protein -
K8U55_RS27530 (K8U55_27525) 45437..45931 + 495 WP_011251274.1 hypothetical protein -
K8U55_RS27535 (K8U55_27530) 45937..46377 + 441 WP_011251275.1 hypothetical protein -
K8U55_RS27540 (K8U55_27535) 46802..47758 - 957 WP_011251280.1 DsbA family protein -
K8U55_RS27545 (K8U55_27540) 47818..48159 - 342 WP_011251281.1 hypothetical protein -
K8U55_RS27550 (K8U55_27545) 48173..48484 - 312 WP_011251282.1 hypothetical protein -
K8U55_RS27555 (K8U55_27550) 48501..48950 - 450 WP_011251283.1 thioredoxin fold domain-containing protein -
K8U55_RS27565 (K8U55_27560) 49798..50268 - 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
K8U55_RS27570 (K8U55_27565) 50265..50708 - 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
K8U55_RS27575 (K8U55_27570) 50809..51312 - 504 Protein_61 DUF4113 domain-containing protein -
K8U55_RS27580 (K8U55_27575) 51519..52499 - 981 Protein_62 IS5-like element ISKpn26 family transposase -
K8U55_RS27585 (K8U55_27580) 52790..53712 + 923 Protein_63 IS5 family transposase -
K8U55_RS27590 (K8U55_27585) 53991..54623 + 633 WP_004213609.1 mucoid phenotype regulator RmpA -
K8U55_RS27595 (K8U55_27590) 54679..54801 + 123 WP_213034833.1 mucoid phenotype synthesis protein RmpD -
K8U55_RS27600 (K8U55_27595) 55154..55555 + 402 WP_214588034.1 LuxR C-terminal-related transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - rmpA / iroN / iucA / iucB / iucC / iucC / iucD / iutA / rmpA2 1..201125 201125
- inside IScluster/Tn - rmpA / iroN 43517..64558 21041


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T219201 WP_004026354.1 NZ_CP084706:c50268-49798 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T219201 NZ_CP116197:2681936-2682043 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT219201 WP_004026357.1 NZ_CP084706:c50708-50265 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT219201 NZ_CP116197:c2681889-2681823 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References