Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 1069922..1070547 Replicon chromosome
Accession NZ_CP084705
Organism Klebsiella pneumoniae strain KP1064WHY

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag K8U55_RS05105 Protein ID WP_002882817.1
Coordinates 1069922..1070305 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag K8U55_RS05110 Protein ID WP_004150355.1
Coordinates 1070305..1070547 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8U55_RS05090 (K8U55_05090) 1067287..1068189 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
K8U55_RS05095 (K8U55_05095) 1068186..1068821 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
K8U55_RS05100 (K8U55_05100) 1068818..1069748 + 931 Protein_976 formate dehydrogenase accessory protein FdhE -
K8U55_RS05105 (K8U55_05105) 1069922..1070305 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
K8U55_RS05110 (K8U55_05110) 1070305..1070547 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
K8U55_RS05115 (K8U55_05115) 1070752..1071669 + 918 WP_002882812.1 alpha/beta hydrolase -
K8U55_RS05120 (K8U55_05120) 1071683..1072624 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
K8U55_RS05125 (K8U55_05125) 1072669..1073106 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
K8U55_RS05130 (K8U55_05130) 1073103..1073963 - 861 WP_002882807.1 virulence factor BrkB family protein -
K8U55_RS05135 (K8U55_05135) 1073957..1074556 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T219186 WP_002882817.1 NZ_CP084705:c1070305-1069922 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T219186 NZ_CP116195:96263-96679 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCAGTGCTTAAGAATCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGTC
CAAAGGCATCGCCACGCCACGTGCAGCTGGTTGACGCGTTCTGCGCCCGTCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGATGCAACCACGGAGGTTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGTCCAAACGACACGGCGATCGC
CGGGCACGCCATCGCCACCGGGGCAATACTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTGCCTGGACTGGTACTGG
AAGACTGGGCAGGATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT219186 WP_004150355.1 NZ_CP084705:c1070547-1070305 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT219186 NZ_CP116195:96036-96266 [Escherichia coli]
ATGAGAACCGTATCTATTTTCAAAAATGGCAATAACCGCGCGATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
TGAGCTGGAGATTGTACGGGAAGGGGACAGAATCATTCTGCGTCCCCTCCGCCCGAGCTGGAGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGGCTTTATGACGGAACGCGAGGACGTTGTCTGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References