Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 29177..29796 Replicon chromosome
Accession NZ_CP084705
Organism Klebsiella pneumoniae strain KP1064WHY

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag K8U55_RS00160 Protein ID WP_002892050.1
Coordinates 29177..29395 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag K8U55_RS00165 Protein ID WP_002892066.1
Coordinates 29422..29796 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8U55_RS00125 (K8U55_00125) 25223..25487 + 265 Protein_24 type B 50S ribosomal protein L31 -
K8U55_RS00130 (K8U55_00130) 25487..25618 + 132 Protein_25 type B 50S ribosomal protein L36 -
K8U55_RS00135 (K8U55_00135) 25625..26323 - 699 WP_226522405.1 GNAT family protein -
K8U55_RS00140 (K8U55_00140) 26424..27874 + 1451 Protein_27 PLP-dependent aminotransferase family protein -
K8U55_RS00145 (K8U55_00145) 27849..28319 - 471 WP_002892026.1 YlaC family protein -
K8U55_RS00150 (K8U55_00150) 28340..28480 + 141 WP_004147370.1 hypothetical protein -
K8U55_RS00155 (K8U55_00155) 28452..29018 - 567 WP_002892030.1 maltose O-acetyltransferase -
K8U55_RS00160 (K8U55_00160) 29177..29395 - 219 WP_002892050.1 HHA domain-containing protein Toxin
K8U55_RS00165 (K8U55_00165) 29422..29796 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
K8U55_RS00170 (K8U55_00170) 30283..33432 - 3150 Protein_33 multidrug efflux RND transporter permease subunit AcrB -
K8U55_RS00175 (K8U55_00175) 33455..34648 - 1194 Protein_34 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T219182 WP_002892050.1 NZ_CP084705:c29395-29177 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T219182 NZ_CP116194:c4036141-4035827 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT219182 WP_002892066.1 NZ_CP084705:c29796-29422 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT219182 NZ_CP116194:c4035830-4035414 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References