Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 11283..11902 Replicon chromosome
Accession NZ_CP084705
Organism Klebsiella pneumoniae strain KP1064WHY

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag K8U55_RS00070 Protein ID WP_002892050.1
Coordinates 11283..11501 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag K8U55_RS00075 Protein ID WP_002892066.1
Coordinates 11528..11902 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8U55_RS00035 (K8U55_00035) 7326..7590 + 265 Protein_6 type B 50S ribosomal protein L31 -
K8U55_RS00040 (K8U55_00040) 7590..7721 + 132 Protein_7 type B 50S ribosomal protein L36 -
K8U55_RS00045 (K8U55_00045) 7728..8426 - 699 WP_226522405.1 GNAT family protein -
K8U55_RS00050 (K8U55_00050) 8527..9980 + 1454 Protein_9 PLP-dependent aminotransferase family protein -
K8U55_RS00055 (K8U55_00055) 9955..10425 - 471 WP_002892026.1 YlaC family protein -
K8U55_RS00060 (K8U55_00060) 10446..10586 + 141 WP_004147370.1 hypothetical protein -
K8U55_RS00065 (K8U55_00065) 10558..11124 - 567 WP_002892030.1 maltose O-acetyltransferase -
K8U55_RS00070 (K8U55_00070) 11283..11501 - 219 WP_002892050.1 HHA domain-containing protein Toxin
K8U55_RS00075 (K8U55_00075) 11528..11902 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
K8U55_RS00080 (K8U55_00080) 12388..15536 - 3149 Protein_15 multidrug efflux RND transporter permease subunit AcrB -
K8U55_RS00085 (K8U55_00085) 15559..16753 - 1195 Protein_16 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T219181 WP_002892050.1 NZ_CP084705:c11501-11283 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T219181 NZ_CP116194:c3971566-3971270 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT219181 WP_002892066.1 NZ_CP084705:c11902-11528 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT219181 NZ_CP116194:c3971268-3970873 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References