Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 496091..496724 Replicon chromosome
Accession NZ_CP084703
Organism Bacillus shivajii strain JCM 32183

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag LGQ02_RS02465 Protein ID WP_096190514.1
Coordinates 496374..496724 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID -
Locus tag LGQ02_RS02460 Protein ID WP_096186805.1
Coordinates 496091..496369 (+) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGQ02_RS02445 (LGQ02_02445) 491461..492999 + 1539 WP_226516672.1 NAD(P)H-hydrate dehydratase -
LGQ02_RS02450 (LGQ02_02450) 493049..494086 + 1038 WP_226516673.1 outer membrane lipoprotein carrier protein LolA -
LGQ02_RS02455 (LGQ02_02455) 494594..495766 + 1173 WP_226516674.1 alanine racemase -
LGQ02_RS02460 (LGQ02_02460) 496091..496369 + 279 WP_096186805.1 antitoxin Antitoxin
LGQ02_RS02465 (LGQ02_02465) 496374..496724 + 351 WP_096190514.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LGQ02_RS02470 (LGQ02_02470) 497080..497904 + 825 WP_226516675.1 RsbT co-antagonist protein RsbRA -
LGQ02_RS02475 (LGQ02_02475) 497919..498275 + 357 WP_226516676.1 STAS domain-containing protein -
LGQ02_RS02480 (LGQ02_02480) 498279..498680 + 402 WP_226516677.1 anti-sigma regulatory factor -
LGQ02_RS02485 (LGQ02_02485) 498716..499708 + 993 WP_226518193.1 PP2C family protein-serine/threonine phosphatase -
LGQ02_RS02490 (LGQ02_02490) 499769..500098 + 330 WP_226516678.1 STAS domain-containing protein -
LGQ02_RS02495 (LGQ02_02495) 500100..500591 + 492 WP_226516679.1 anti-sigma B factor RsbW -
LGQ02_RS02500 (LGQ02_02500) 500554..501342 + 789 WP_226516680.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12921.03 Da        Isoelectric Point: 6.4662

>T219174 WP_096190514.1 NZ_CP084703:496374-496724 [Bacillus shivajii]
MIVKRGDVYFADLSPVVGSEQGGIRPVLIIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEINAKRYGFDRDSVILLEQV
RTIDKQRLTDKITHLDDDMMAKVNDALMISLGLIDF

Download         Length: 351 bp

>T219174 NZ_CP116194:c1986348-1986241 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10702.20 Da        Isoelectric Point: 4.8895

>AT219174 WP_096186805.1 NZ_CP084703:496091-496369 [Bacillus shivajii]
VSEENTKKIMVSLPQHLVNELDGLLERQNVNRSEIIHQATKMYLREQKKQQIRETMQQGYMEMAKINLNIATESFLVEEE
AENTLDRLVSGV

Download         Length: 279 bp

>AT219174 NZ_CP116194:1986401-1986462 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References