Detailed information of TA system TA219171

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 272989..273665
• Replicon: plasmid pRL12
• Accession: NZ_CP084699
• Organism: Rhizobium ruizarguesonis strain RCAM 1026

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BSO17_RS31490
• Protein ID: WP_024321537.1
• Coordinates: 273240..273665 (+)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A4Q8ZXM1
• Locus tag: BSO17_RS31485
• Protein ID: WP_075224380.1
• Coordinates: 272989..273243 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BSO17_RS31455 (BSO17_31470)	268705..269598	-	894	WP_024321543.1	NmrA family NAD(P)-binding protein	-
BSO17_RS31460 (BSO17_31475)	269668..270090	-	423	WP_024321542.1	MarR family winged helix-turn-helix transcriptional regulator	-
BSO17_RS31465 (BSO17_31480)	270112..270324	+	213	WP_226532357.1	hypothetical protein	-
BSO17_RS31470 (BSO17_31485)	270399..271370	-	972	WP_024321540.1	alpha/beta hydrolase	-
BSO17_RS31475 (BSO17_31490)	271467..272153	-	687	WP_024321539.1	glutathione S-transferase	-
BSO17_RS31480 (BSO17_31495)	272258..272833	+	576	WP_029768016.1	TetR/AcrR family transcriptional regulator	-
BSO17_RS31485 (BSO17_31500)	272989..273243	+	255	WP_075224380.1	plasmid stabilization protein	Antitoxin
BSO17_RS31490 (BSO17_31505)	273240..273665	+	426	WP_024321537.1	type II toxin-antitoxin system VapC family toxin	Toxin
BSO17_RS31495 (BSO17_31510)	274111..274899	+	789	WP_024321536.1	siderophore-iron reductase FhuF	-
BSO17_RS31500 (BSO17_31515)	275016..275693	-	678	Protein_260	phosphopantetheinyl transferase	-
BSO17_RS31505 (BSO17_31520)	276080..277957	+	1878	WP_024321535.1	HAMP domain-containing methyl-accepting chemotaxis protein	-
BSO17_RS31510 (BSO17_31525)	278154..278543	+	390	WP_024321534.1	RidA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..838366	838366

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 15214.47 Da
• Isoelectric Point: 4.8283

>T219171 WP_024321537.1 NZ_CP084699:273240-273665 [Rhizobium ruizarguesonis]
MILIDTNVISEPWKPTPDNQVLAWIDVQAVETLFVSAITVAELRFGIAAMPSGRRKTVLDDRLEQEVLPLFSGRVLSFDM
AASQSYSELMARARAAGRAIGKADAYIAATAAANGFIVATRDSSPFEAAGLKVINPWYAPT

Nucleotide

• Length: 426 bp

>T219171 NZ_CP116194:1003654-1004052 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGTGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGCAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9249.43 Da
• Isoelectric Point: 5.6158

>AT219171 WP_075224380.1 NZ_CP084699:272989-273243 [Rhizobium ruizarguesonis]
MAAVTIRNLSDETHRALRVRAAHHGRSTEAEIRDILETAVRPAERVMLGSLLASIGREAGLSNDDVDNLQLVRDKTPAEP
MSFE

Nucleotide

• Length: 255 bp

>AT219171 NZ_CP116194:1003358-1003651 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
TGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A4Q8ZXM1