Detailed information of TA system TA219149

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 3615234..3615872
• Replicon: chromosome
• Accession: NZ_CP084683
• Organism: Escherichia coli strain BL21(RP)

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: LGS58_RS17440
• Protein ID: WP_000813794.1
• Coordinates: 3615234..3615410 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LGS58_RS17445
• Protein ID: WP_001270286.1
• Coordinates: 3615456..3615872 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGS58_RS17420 (3610853)	3610853..3612028	-	1176	WP_001236265.1	BenE family transporter YdcO	-
LGS58_RS17425 (3612120)	3612120..3612656	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
LGS58_RS17430 (3612729)	3612729..3614690	+	1962	WP_001301045.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
LGS58_RS17435 (3614782)	3614782..3615012	-	231	WP_000494244.1	YncJ family protein	-
LGS58_RS17440 (3615234)	3615234..3615410	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
LGS58_RS17445 (3615456)	3615456..3615872	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
LGS58_RS17450 (3615951)	3615951..3617357	+	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
LGS58_RS17455 (3617602)	3617602..3618747	+	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
LGS58_RS17460 (3618765)	3618765..3619778	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
LGS58_RS17465 (3619779)	3619779..3620720	+	942	WP_001251313.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T219149 WP_000813794.1 NZ_CP084683:3615234-3615410 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T219149 NZ_CP116191:c1203300-1203082 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT219149 WP_001270286.1 NZ_CP084683:3615456-3615872 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT219149 NZ_CP116191:c1203700-1203326 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• PDB: 6HPB