Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 3615234..3615872 Replicon chromosome
Accession NZ_CP084683
Organism Escherichia coli strain BL21(RP)

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag LGS58_RS17440 Protein ID WP_000813794.1
Coordinates 3615234..3615410 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag LGS58_RS17445 Protein ID WP_001270286.1
Coordinates 3615456..3615872 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGS58_RS17420 (3610853) 3610853..3612028 - 1176 WP_001236265.1 BenE family transporter YdcO -
LGS58_RS17425 (3612120) 3612120..3612656 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
LGS58_RS17430 (3612729) 3612729..3614690 + 1962 WP_001301045.1 23S rRNA 5-hydroxycytidine C2501 synthase -
LGS58_RS17435 (3614782) 3614782..3615012 - 231 WP_000494244.1 YncJ family protein -
LGS58_RS17440 (3615234) 3615234..3615410 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
LGS58_RS17445 (3615456) 3615456..3615872 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
LGS58_RS17450 (3615951) 3615951..3617357 + 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
LGS58_RS17455 (3617602) 3617602..3618747 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
LGS58_RS17460 (3618765) 3618765..3619778 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
LGS58_RS17465 (3619779) 3619779..3620720 + 942 WP_001251313.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T219149 WP_000813794.1 NZ_CP084683:3615234-3615410 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T219149 NZ_CP116191:c1203300-1203082 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT219149 WP_001270286.1 NZ_CP084683:3615456-3615872 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT219149 NZ_CP116191:c1203700-1203326 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References