Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 2982055..2982892 Replicon chromosome
Accession NZ_CP084683
Organism Escherichia coli strain BL21(RP)

Toxin (Protein)


Gene name itaT Uniprot ID A0A140NB16
Locus tag LGS58_RS14360 Protein ID WP_000227786.1
Coordinates 2982350..2982892 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag LGS58_RS14355 Protein ID WP_001297137.1
Coordinates 2982055..2982366 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGS58_RS14330 (2977075) 2977075..2978022 + 948 WP_001239440.1 cytochrome o ubiquinol oxidase subunit II -
LGS58_RS14335 (2978044) 2978044..2980035 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
LGS58_RS14340 (2980025) 2980025..2980639 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
LGS58_RS14345 (2980639) 2980639..2980968 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
LGS58_RS14350 (2980980) 2980980..2981870 + 891 WP_000971336.1 heme o synthase -
LGS58_RS14355 (2982055) 2982055..2982366 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
LGS58_RS14360 (2982350) 2982350..2982892 + 543 WP_000227786.1 GNAT family N-acetyltransferase Toxin
LGS58_RS14365 (2982948) 2982948..2983883 - 936 WP_001297127.1 tetratricopeptide repeat protein -
LGS58_RS14370 (2984300) 2984300..2985664 + 1365 WP_001000978.1 MFS transporter -
LGS58_RS14375 (2985792) 2985792..2986283 - 492 WP_001138904.1 nucleotide binding protein YajQ -
LGS58_RS14380 (2986451) 2986451..2987362 + 912 WP_000705853.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19794.98 Da        Isoelectric Point: 8.3395

>T219147 WP_000227786.1 NZ_CP084683:2982350-2982892 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADSAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T219147 NZ_CP116191:728696-729010 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT219147 WP_001297137.1 NZ_CP084683:2982055-2982366 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT219147 NZ_CP116191:728460-728693 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACA
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A140NB16

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References