Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2666760..2667378 Replicon chromosome
Accession NZ_CP084683
Organism Escherichia coli strain BL21(RP)

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag LGS58_RS12835 Protein ID WP_001291435.1
Coordinates 2667160..2667378 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag LGS58_RS12830 Protein ID WP_000344800.1
Coordinates 2666760..2667134 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGS58_RS12820 (2661849) 2661849..2663042 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LGS58_RS12825 (2663065) 2663065..2666214 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
LGS58_RS12830 (2666760) 2666760..2667134 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
LGS58_RS12835 (2667160) 2667160..2667378 + 219 WP_001291435.1 HHA domain-containing protein Toxin
LGS58_RS12840 (2667550) 2667550..2668101 + 552 WP_000102564.1 maltose O-acetyltransferase -
LGS58_RS12845 (2668217) 2668217..2668687 + 471 WP_000136192.1 YlaC family protein -
LGS58_RS12850 (2668851) 2668851..2670401 + 1551 WP_001319841.1 cyclic-guanylate-specific phosphodiesterase PdeB -
LGS58_RS12855 (2670443) 2670443..2670796 - 354 WP_000878140.1 DUF1428 domain-containing protein -
LGS58_RS12865 (2671175) 2671175..2671486 + 312 WP_000409911.1 MGMT family protein -
LGS58_RS12870 (2671517) 2671517..2672089 - 573 WP_000779826.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T219145 WP_001291435.1 NZ_CP084683:2667160-2667378 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T219145 NZ_CP116191:c612487-612146 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACTGCGGTAGATGTCAAAGTAATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTCGCCTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT219145 WP_000344800.1 NZ_CP084683:2666760-2667134 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT219145 NZ_CP116191:612482-612558 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References