Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1748200..1748854 Replicon chromosome
Accession NZ_CP084683
Organism Escherichia coli strain BL21(RP)

Toxin (Protein)


Gene name cptA Uniprot ID A0A140N853
Locus tag LGS58_RS08515 Protein ID WP_000244783.1
Coordinates 1748447..1748854 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag LGS58_RS08510 Protein ID WP_000354046.1
Coordinates 1748200..1748466 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGS58_RS08485 (1743369) 1743369..1744112 + 744 WP_000951948.1 SDR family oxidoreductase -
LGS58_RS08490 (1744169) 1744169..1745602 - 1434 WP_012775976.1 6-phospho-beta-glucosidase BglA -
LGS58_RS08495 (1745647) 1745647..1745958 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
LGS58_RS08500 (1746122) 1746122..1746781 + 660 WP_000250274.1 hemolysin III family protein -
LGS58_RS08505 (1746977) 1746977..1747957 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
LGS58_RS08510 (1748200) 1748200..1748466 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
LGS58_RS08515 (1748447) 1748447..1748854 + 408 WP_000244783.1 protein YgfX Toxin
LGS58_RS08520 (1748894) 1748894..1749415 - 522 WP_001055874.1 flavodoxin FldB -
LGS58_RS08525 (1749527) 1749527..1750423 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
LGS58_RS08530 (1750448) 1750448..1751158 + 711 WP_000715216.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LGS58_RS08535 (1751164) 1751164..1752897 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15962.85 Da        Isoelectric Point: 11.2669

>T219141 WP_000244783.1 NZ_CP084683:1748447-1748854 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEISLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T219141 NZ_CP116189:73711-73860 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGAGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT219141 WP_000354046.1 NZ_CP084683:1748200-1748466 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT219141 NZ_CP116189:c73667-73606 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A140N853

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References