Detailed information of TA system TA219130

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): lsoAB/RnlA-RnlB
• Location: 428..1845
• Replicon: plasmid unnamed3
• Accession: NZ_CP084667
• Organism: UNVERIFIED_ORG: Hafnia paralvei strain FDAARGOS_1502

Toxin (Protein)

• Gene name: lsoA
• Uniprot ID: -
• Locus tag: LA328_RS22270
• Protein ID: WP_047502540.1
• Coordinates: 428..1468 (+)
• Length: 347 a.a.

Antitoxin (Protein)

• Gene name: lsoB
• Uniprot ID: -
• Locus tag: LA328_RS22275
• Protein ID: WP_047502545.1
• Coordinates: 1465..1845 (+)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LA328_RS22270 (LA328_22270)	428..1468	+	1041	WP_047502540.1	type II toxin-antitoxin system RnlA family toxin	Toxin
LA328_RS22275 (LA328_22275)	1465..1845	+	381	WP_047502545.1	type II toxin-antitoxin system RnlB family antitoxin	Antitoxin
LA328_RS22280 (LA328_22280)	2054..2539	-	486	WP_226229144.1	hypothetical protein	-
LA328_RS22285 (LA328_22285)	2716..2925	-	210	WP_072034108.1	MbeD family mobilization/exclusion protein	-
LA328_RS22290 (LA328_22290)	2929..3414	-	486	WP_072034109.1	MbeB family mobilization protein	-
LA328_RS22295 (LA328_22295)	3449..3883	-	435	Protein_5	relaxase/mobilization nuclease domain-containing protein	-
LA328_RS22300 (LA328_22300)	3812..4105	+	294	Protein_6	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..4105	4105

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Bacterial toxin, RNase RnlA/LsoA, N-terminal (6-80)

Antitoxin

• Antitoxin RnlB/LsoB (20-114)

Sequences

Toxin

Protein

• Length: 347 a.a.
• Molecular weight: 39595.82 Da
• Isoelectric Point: 5.1769

>T219130 WP_047502540.1 NZ_CP084667:428-1468 [Hafnia paralvei]
MAQNPFKALNINTDKIEAALTQNGVTNFSSTVKNERETHLSGTYKEIDFLIKLMPSGGNTTIGKASGQNNTYFDEIAGII
KENCLYSDKKTFEYTIPRFSDENKEMLFSFLAEENVKIEEDQNNDPNCKHQYIMTTGNGDRVRAKIYNRGSIQFQGKYLQ
IASLINDFMCTILDMKDIIKQKNQEFNVDIKKETIESELHSKLPKSIDQIHDDIKKQLSCSLIMKKIDVEMEDYSTYCFS
ALRSVEGFIYQLLGSICNPSSSRNLGEYFTENNPKYIIRDIHQETINGEIAEVLCECYTYWHENRHGLFHMKPGVADTKT
IDKAESIEIIDTVCQLIDNGIARLKS

Nucleotide

• Length: 1041 bp

>T219130 NZ_CP116188:2956491-2956865 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 14520.75 Da
• Isoelectric Point: 10.0166

>AT219130 WP_047502545.1 NZ_CP084667:1465-1845 [Hafnia paralvei]
MKINKKYQIEVMKDKDKTLFIVYATDIYSPSEFFSKIESDLKKKKVGGDVFFDLIIPNGGKKDRYVYTSFNGERLTSYIL
NKVTETDEYKSLSERSASFFKKNFDKINANLLSKATSFALKQGIPI

Nucleotide

• Length: 381 bp

>AT219130 NZ_CP116188:2956034-2956402 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGGGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA