Detailed information of TA system TA021913

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-PumB/upstrm_HI1419-dnstrm_HI1420
• Location: 4283323..4283943
• Replicon: chromosome
• Accession: NC_008781
• Organism: Polaromonas naphthalenivorans CJ2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: PNAP_RS20010
• Protein ID: WP_041377499.1
• Coordinates: 4283323..4283619 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A1VUI2
• Locus tag: PNAP_RS20015
• Protein ID: WP_011803374.1
• Coordinates: 4283623..4283943 (+)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PNAP_RS25150	4278440..4281310	+	2871	WP_011803370.1	filamentous hemagglutinin N-terminal domain-containing protein	-
PNAP_RS20000	4281347..4282174	+	828	WP_157040340.1	hypothetical protein	-
PNAP_RS20005	4282221..4283174	-	954	WP_011803372.1	2-dehydropantoate 2-reductase	-
PNAP_RS20010	4283323..4283619	+	297	WP_041377499.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PNAP_RS20015	4283623..4283943	+	321	WP_011803374.1	putative addiction module antidote protein	Antitoxin
PNAP_RS20020	4284159..4285505	+	1347	WP_011803375.1	NAD(P)-dependent oxidoreductase	-
PNAP_RS20025	4285556..4286863	+	1308	WP_011803376.1	NAD-dependent dihydropyrimidine dehydrogenase subunit PreA	-
PNAP_RS20030	4286928..4288373	+	1446	WP_011803377.1	dihydropyrimidinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-94)

Antitoxin

• Uncharacterized protein HI1420 (11-99)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10679.34 Da
• Isoelectric Point: 9.1442

>T21913 WP_041377499.1 NC_008781:4283323-4283619 [Polaromonas naphthalenivorans CJ2]
MLQVLRTHEFDAWISGLRDKVGQKQVLARLARLSLGHWGDCKPVGGEVTELRIDSGPGYRVYCWKDGATVVVALGGGDKS
GQDKDIAKAQGMVKLLKE

Nucleotide

• Length: 297 bp

>T21913 NC_008781:4283323-4283619 [Polaromonas naphthalenivorans CJ2]
ATGCTACAAGTCCTGCGCACCCATGAGTTCGATGCCTGGATCAGCGGGCTTCGTGACAAGGTCGGGCAAAAGCAGGTTCT
GGCGCGTCTTGCCCGTCTCTCGCTGGGCCACTGGGGCGACTGCAAGCCTGTTGGCGGCGAAGTCACCGAACTTCGCATTG
ACTCAGGCCCCGGCTACCGCGTCTATTGCTGGAAAGACGGTGCCACGGTCGTTGTTGCCTTGGGCGGGGGCGACAAGTCT
GGCCAGGACAAAGACATTGCGAAGGCCCAAGGCATGGTCAAACTTTTGAAGGAGTGA

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 11455.01 Da
• Isoelectric Point: 4.4720

>AT21913 WP_011803374.1 NC_008781:4283623-4283943 [Polaromonas naphthalenivorans CJ2]
MNPTAAEKLGIQPFDAADYLQSDEDCAMYLQACIEEAPDDAALFARALGDIARARGMMQLARDTGITREGLYKALGEQGN
PSLSTVLKVLHALGLRLQVDPRPDHA

Nucleotide

• Length: 321 bp

>AT21913 NC_008781:4283623-4283943 [Polaromonas naphthalenivorans CJ2]
ATGAACCCCACAGCCGCCGAAAAACTGGGCATCCAGCCCTTCGATGCCGCCGACTACCTGCAGTCCGATGAAGACTGCGC
CATGTACCTGCAGGCCTGCATTGAAGAGGCGCCTGACGATGCCGCCCTGTTTGCCAGGGCGCTGGGCGATATCGCCCGCG
CGCGCGGCATGATGCAGCTCGCCCGCGACACCGGAATCACCCGCGAAGGACTCTACAAGGCCTTGGGCGAGCAAGGCAAC
CCGAGCCTTTCCACGGTGCTGAAGGTGCTGCACGCCCTTGGCCTGCGGCTGCAGGTTGATCCACGGCCGGACCACGCATA
A

Structures

Antitoxin

• AlphaFold DB: A1VUI2