Detailed information of TA system TA021912

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yoeB-relE/YoeB-YefM
• Location: 4169816..4170318
• Replicon: chromosome
• Accession: NC_008781
• Organism: Polaromonas naphthalenivorans CJ2

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A1VU72
• Locus tag: PNAP_RS19510
• Protein ID: WP_011803266.1
• Coordinates: 4169816..4170070 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: relE
• Uniprot ID: A1VU73
• Locus tag: PNAP_RS19515
• Protein ID: WP_011803267.1
• Coordinates: 4170067..4170318 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PNAP_RS19495	4164947..4166137	+	1191	WP_011803263.1	efflux RND transporter periplasmic adaptor subunit	-
PNAP_RS19500	4166134..4169268	+	3135	WP_011803264.1	efflux RND transporter permease subunit	-
PNAP_RS19505	4169319..4169576	+	258	WP_011803265.1	hypothetical protein	-
PNAP_RS19510	4169816..4170070	-	255	WP_011803266.1	Txe/YoeB family addiction module toxin	Toxin
PNAP_RS19515	4170067..4170318	-	252	WP_011803267.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
PNAP_RS19520	4170576..4171514	-	939	WP_011803268.1	LysR family transcriptional regulator	-
PNAP_RS19525	4171613..4172620	+	1008	WP_011803269.1	tripartite tricarboxylate transporter substrate binding protein	-
PNAP_RS19530	4172635..4173705	+	1071	WP_041377483.1	maleylacetate reductase	-
PNAP_RS19535	4173805..4174800	+	996	WP_011803271.1	NAD(P)H-quinone oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10189.70 Da
• Isoelectric Point: 9.3592

>T21912 WP_011803266.1 NC_008781:c4170070-4169816 [Polaromonas naphthalenivorans CJ2]
VKLVFADEAWEDYLYWQKHDKRMVERINKLIRETQREPFSGVGKPEPLKHALSGFWSRRITDEHRMVYKVEGDALLLAQL
RYHY

Nucleotide

• Length: 255 bp

>T21912 NC_008781:c4170070-4169816 [Polaromonas naphthalenivorans CJ2]
GTGAAGCTGGTCTTTGCGGATGAGGCTTGGGAAGACTACCTCTACTGGCAAAAGCACGACAAGCGGATGGTTGAGCGCAT
CAACAAGCTCATCCGGGAGACACAGCGCGAACCGTTCTCGGGTGTCGGCAAGCCCGAGCCGCTCAAGCATGCGCTGTCAG
GCTTCTGGTCGCGCCGGATTACCGATGAACATCGCATGGTTTACAAGGTAGAAGGCGATGCGCTTTTGCTGGCGCAGCTT
CGTTACCACTATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 8995.15 Da
• Isoelectric Point: 4.9690

>AT21912 WP_011803267.1 NC_008781:c4170318-4170067 [Polaromonas naphthalenivorans CJ2]
MNAITYSAARANLASTMNRVCNDHEALIITRNGQQSVVMLSLEDFQALEETAYLLRSPANAKRLLAAAAQLTAGQGTERE
LSE

Nucleotide

• Length: 252 bp

>AT21912 NC_008781:c4170318-4170067 [Polaromonas naphthalenivorans CJ2]
ATGAATGCCATCACCTACTCTGCCGCCCGCGCCAACCTGGCCAGCACCATGAATCGGGTGTGCAACGACCATGAAGCCTT
GATCATCACCCGCAACGGACAACAATCCGTGGTCATGCTCTCGCTGGAGGACTTCCAGGCGCTGGAAGAAACGGCCTACC
TGCTGCGCAGCCCCGCCAACGCCAAGCGCCTGCTCGCTGCCGCTGCCCAACTCACCGCTGGCCAAGGCACTGAGCGGGAG
TTGAGCGAGTGA

Structures

Toxin

• AlphaFold DB: A1VU72

Antitoxin

• AlphaFold DB: A1VU73