Detailed information of TA system TA219112

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 3446963..3447489
• Replicon: chromosome
• Accession: NZ_CP084656
• Organism: Pectobacterium versatile strain SR1

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: LGL96_RS15330
• Protein ID: WP_225586011.1
• Coordinates: 3446963..3447268 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: C6C380
• Locus tag: LGL96_RS15335
• Protein ID: WP_015855076.1
• Coordinates: 3447271..3447489 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGL96_RS15300 (LGL96_15300)	3442159..3442407	-	249	WP_225586013.1	helix-turn-helix transcriptional regulator	-
LGL96_RS15305 (LGL96_15305)	3442414..3442556	+	143	Protein_2973	helix-turn-helix domain-containing protein	-
LGL96_RS15310 (LGL96_15310)	3442895..3443188	+	294	WP_161128727.1	DUF1778 domain-containing protein	-
LGL96_RS15315 (LGL96_15315)	3443185..3443679	+	495	WP_130621813.1	GNAT family N-acetyltransferase	-
LGL96_RS15320 (LGL96_15320)	3444105..3445835	+	1731	WP_130621814.1	AAA family ATPase	-
LGL96_RS15325 (LGL96_15325)	3445921..3446763	+	843	WP_130621815.1	DNA adenine methylase	-
LGL96_RS15330 (LGL96_15330)	3446963..3447268	-	306	WP_225586011.1	type II toxin-antitoxin system toxin CcdB	Toxin
LGL96_RS15335 (LGL96_15335)	3447271..3447489	-	219	WP_015855076.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
LGL96_RS15340 (LGL96_15340)	3447548..3448291	-	744	WP_225586009.1	MobC family replication-relaxation protein	-
LGL96_RS15345 (LGL96_15345)	3449190..3449531	-	342	WP_225586008.1	hypothetical protein	-
LGL96_RS15350 (LGL96_15350)	3449718..3449912	+	195	WP_012773549.1	YlcI/YnfO family protein	-
LGL96_RS15355 (LGL96_15355)	3450007..3451266	-	1260	WP_225586007.1	integrase arm-type DNA-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3427202..3451362	24160

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11758.64 Da
• Isoelectric Point: 4.4964

>T219112 WP_225586011.1 NZ_CP084656:c3447268-3446963 [Pectobacterium versatile]
MQFIVYQYTRASHYKMFVDVQSDIVETPKRRMVIPIIEAYHLSEKVNKTLFPLIRIDAEDYRLMTTELSSVPVEVIGEVI
ADLGDYADEIKDAINLMFWGI

Nucleotide

• Length: 306 bp

>T219112 NZ_CP116181:c4447435-4447262 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTTTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8178.13 Da
• Isoelectric Point: 7.0135

>AT219112 WP_015855076.1 NZ_CP084656:c3447489-3447271 [Pectobacterium versatile]
MKHRVSVTVDKDNYQVLSAAGVNISGLVNDAIGKEARRIKAEEWKKENREGMEEVARFIAQNGSFADENRNW

Nucleotide

• Length: 219 bp

>AT219112 NZ_CP116181:c4447759-4447463 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A250BG46