Detailed information of TA system TA219111

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3442895..3443679
• Replicon: chromosome
• Accession: NZ_CP084656
• Organism: Pectobacterium versatile strain SR1

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: LGL96_RS15315
• Protein ID: WP_130621813.1
• Coordinates: 3443185..3443679 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: LGL96_RS15310
• Protein ID: WP_161128727.1
• Coordinates: 3442895..3443188 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGL96_RS15275 (LGL96_15275)	3438892..3439299	+	408	WP_039490298.1	hypothetical protein	-
LGL96_RS15280 (LGL96_15280)	3439338..3440786	+	1449	WP_225586017.1	DUF4832 domain-containing protein	-
LGL96_RS15285 (LGL96_15285)	3440813..3441313	+	501	WP_103861241.1	serine acetyltransferase	-
LGL96_RS15290 (LGL96_15290)	3441520..3441805	+	286	Protein_2970	DUF1778 domain-containing protein	-
LGL96_RS15295 (LGL96_15295)	3441926..3442144	-	219	WP_225586015.1	type II toxin-antitoxin system antitoxin CcdA	-
LGL96_RS15300 (LGL96_15300)	3442159..3442407	-	249	WP_225586013.1	helix-turn-helix transcriptional regulator	-
LGL96_RS15305 (LGL96_15305)	3442414..3442556	+	143	Protein_2973	helix-turn-helix domain-containing protein	-
LGL96_RS15310 (LGL96_15310)	3442895..3443188	+	294	WP_161128727.1	DUF1778 domain-containing protein	Antitoxin
LGL96_RS15315 (LGL96_15315)	3443185..3443679	+	495	WP_130621813.1	GNAT family N-acetyltransferase	Toxin
LGL96_RS15320 (LGL96_15320)	3444105..3445835	+	1731	WP_130621814.1	AAA family ATPase	-
LGL96_RS15325 (LGL96_15325)	3445921..3446763	+	843	WP_130621815.1	DNA adenine methylase	-
LGL96_RS15330 (LGL96_15330)	3446963..3447268	-	306	WP_225586011.1	type II toxin-antitoxin system toxin CcdB	-
LGL96_RS15335 (LGL96_15335)	3447271..3447489	-	219	WP_015855076.1	type II toxin-antitoxin system antitoxin CcdA	-
LGL96_RS15340 (LGL96_15340)	3447548..3448291	-	744	WP_225586009.1	MobC family replication-relaxation protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3427202..3451362	24160

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (76-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 17653.50 Da
• Isoelectric Point: 7.8051

>T219111 WP_130621813.1 NZ_CP084656:3443185-3443679 [Pectobacterium versatile]
MISAPEPLHAEHVLSSFCCGVESMDNWLKQRAMKNQVTGASRTFVSCDGSKVLAYYSLASSAVATNAAPGRFRRNMPDPI
PVVVLGRLAVDRSLHGQGVGRALVRDAGLRVIQVADTIGIRGMLVHALSDEAREFYLRVGFEPSPIDPMMLMVTLGDLVG
SLSI

Nucleotide

• Length: 495 bp

>T219111 NZ_CP116181:c4344511-4344161 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10914.52 Da
• Isoelectric Point: 10.1968

>AT219111 WP_161128727.1 NZ_CP084656:3442895-3443188 [Pectobacterium versatile]
MPVTNSVSVKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIDQRIIMADPDAYQEFLARLDQAPAPN
ATLRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT219111 NZ_CP116181:c4344756-4344505 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA