Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 2615861..2616478 Replicon chromosome
Accession NZ_CP084656
Organism Pectobacterium versatile strain SR1

Toxin (Protein)


Gene name hicA Uniprot ID C6DHN3
Locus tag LGL96_RS11545 Protein ID WP_010281507.1
Coordinates 2616299..2616478 (-) Length 60 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A426IL41
Locus tag LGL96_RS11540 Protein ID WP_039545938.1
Coordinates 2615861..2616271 (-) Length 137 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGL96_RS11525 (LGL96_11525) 2611725..2612768 - 1044 WP_010299291.1 DNA-binding transcriptional regulator CytR -
LGL96_RS11530 (LGL96_11530) 2613078..2615276 - 2199 WP_039474910.1 primosomal protein N' -
LGL96_RS11535 (LGL96_11535) 2615548..2615763 + 216 WP_010281510.1 50S ribosomal protein L31 -
LGL96_RS11540 (LGL96_11540) 2615861..2616271 - 411 WP_039545938.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
LGL96_RS11545 (LGL96_11545) 2616299..2616478 - 180 WP_010281507.1 type II toxin-antitoxin system HicA family toxin Toxin
LGL96_RS11550 (LGL96_11550) 2616578..2617588 - 1011 WP_039545936.1 iron ABC transporter permease -
LGL96_RS11555 (LGL96_11555) 2617585..2618547 - 963 WP_039545934.1 ABC transporter substrate-binding protein -
LGL96_RS11560 (LGL96_11560) 2618557..2619354 - 798 WP_103943591.1 ABC transporter ATP-binding protein -
LGL96_RS11565 (LGL96_11565) 2619578..2619895 - 318 WP_005973355.1 met regulon transcriptional regulator MetJ -
LGL96_RS11570 (LGL96_11570) 2620083..2621243 + 1161 WP_010299271.1 cystathionine gamma-synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-128)


Sequences

Toxin        


Download         Length: 60 a.a.        Molecular weight: 6560.69 Da        Isoelectric Point: 10.7838

>T219100 WP_010281507.1 NZ_CP084656:c2616478-2616299 [Pectobacterium versatile]
MDSRTLIAEIKADGWELIRVNGSHHHFMHPSKPGLVTIPHPKKDLPIGTVKSIRKQAGI

Download         Length: 180 bp

>T219100 NZ_CP116181:1892174-1892425 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 137 a.a.        Molecular weight: 14533.41 Da        Isoelectric Point: 4.3391

>AT219100 WP_039545938.1 NZ_CP084656:c2616271-2615861 [Pectobacterium versatile]
MFYPIAIEAGDDTHAYGVTVPDLPGCFSAGDTLDDAIANAKEAITGHIELLIEMGQDIPTVSSVGQLAKNAEYAGYTWAV
VDIDVTRLMGGSEKINVTLPKSLIDRIDRCVASNPEFKSRSGFLAQAALERISSSR

Download         Length: 411 bp

>AT219100 NZ_CP116181:1892422-1892676 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W2I1S0

Antitoxin

Source ID Structure
AlphaFold DB A0A426IL41

References