Detailed information of TA system TA219092

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 813763..814397
• Replicon: chromosome
• Accession: NZ_CP084656
• Organism: Pectobacterium versatile strain SR1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LGL96_RS03475
• Protein ID: WP_116156145.1
• Coordinates: 813993..814397 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A1V2R781
• Locus tag: LGL96_RS03470
• Protein ID: WP_039360473.1
• Coordinates: 813763..813993 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGL96_RS03430 (LGL96_03430)	809187..809885	+	699	WP_107333349.1	DeoR family transcriptional regulator	-
LGL96_RS03435 (LGL96_03435)	810065..810637	+	573	WP_225591977.1	hypothetical protein	-
LGL96_RS03440 (LGL96_03440)	810673..811146	+	474	WP_130622704.1	hypothetical protein	-
LGL96_RS03445 (LGL96_03445)	811163..811396	+	234	WP_040034648.1	membrane protein	-
LGL96_RS03450 (LGL96_03450)	811481..811954	+	474	WP_130622703.1	DNA repair protein RadC	-
LGL96_RS03455 (LGL96_03455)	811994..812329	+	336	WP_130622702.1	type IV toxin-antitoxin system YeeU family antitoxin	-
LGL96_RS03460 (LGL96_03460)	812398..812718	+	321	WP_116156147.1	TA system toxin CbtA family protein	-
LGL96_RS03465 (LGL96_03465)	812836..813669	+	834	WP_116156146.1	DUF4942 domain-containing protein	-
LGL96_RS03470 (LGL96_03470)	813763..813993	+	231	WP_039360473.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
LGL96_RS03475 (LGL96_03475)	813993..814397	+	405	WP_116156145.1	tRNA(fMet)-specific endonuclease VapC	Toxin
LGL96_RS03480 (LGL96_03480)	814736..814882	+	147	Protein_683	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
LGL96_RS03485 (LGL96_03485)	814973..816406	-	1434	WP_225586101.1	FAD-binding protein	-
LGL96_RS03490 (LGL96_03490)	816403..817281	-	879	WP_225586102.1	TauD/TfdA family dioxygenase	-
LGL96_RS03495 (LGL96_03495)	817508..817729	-	222	WP_010300175.1	acyl carrier protein	-
LGL96_RS03500 (LGL96_03500)	817746..819179	-	1434	WP_119880018.1	AMP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-53)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15080.21 Da
• Isoelectric Point: 7.1618

>T219092 WP_116156145.1 NZ_CP084656:813993-814397 [Pectobacterium versatile]
MLKYLLDTNICIYTIKNKPQEVRDAFYRYYGQFAISSITLMELIYGAEKSVNTEKNLAVIEGFSARLEVQNYGFDAAVHT
GQIRAELARQGTPIGPYDAMLAGHARSAGLILVTNNVREFERVSGLRVENWVSQ

Nucleotide

• Length: 405 bp

>T219092 NZ_CP116178:14783-15064 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGGGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8707.79 Da
• Isoelectric Point: 4.4091

>AT219092 WP_039360473.1 NZ_CP084656:813763-813993 [Pectobacterium versatile]
MEKTTVFKSNRSQAVRLPKAVALPDDVKQVDIVAIGRTRIITPAGESWDSWFEEQGVTPDFMITREQPDDQIREDF

Nucleotide

• Length: 231 bp

>AT219092 NZ_CP116178:14542-14793 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Structures

Antitoxin

• AlphaFold DB: A0A1V2R781