Detailed information of TA system TA219087

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2091217..2091834
• Replicon: chromosome
• Accession: NZ_CP084655
• Organism: Pectobacterium brasiliense strain SR10

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: C6DHN3
• Locus tag: LGL97_RS09330
• Protein ID: WP_010281507.1
• Coordinates: 2091655..2091834 (-)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LGL97_RS09325
• Protein ID: WP_225583654.1
• Coordinates: 2091217..2091627 (-)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGL97_RS09310 (LGL97_09310)	2087097..2088140	-	1044	WP_225583656.1	DNA-binding transcriptional regulator CytR	-
LGL97_RS09315 (LGL97_09315)	2088449..2090647	-	2199	WP_225583655.1	primosomal protein N'	-
LGL97_RS09320 (LGL97_09320)	2090920..2091135	+	216	WP_010281510.1	50S ribosomal protein L31	-
LGL97_RS09325 (LGL97_09325)	2091217..2091627	-	411	WP_225583654.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LGL97_RS09330 (LGL97_09330)	2091655..2091834	-	180	WP_010281507.1	type II toxin-antitoxin system HicA family toxin	Toxin
LGL97_RS09335 (LGL97_09335)	2091918..2092928	-	1011	WP_225583649.1	iron ABC transporter permease	-
LGL97_RS09340 (LGL97_09340)	2092929..2093888	-	960	WP_225583647.1	ABC transporter substrate-binding protein	-
LGL97_RS09345 (LGL97_09345)	2093898..2094695	-	798	WP_225583646.1	ABC transporter ATP-binding protein	-
LGL97_RS09350 (LGL97_09350)	2094930..2095247	-	318	WP_005973355.1	met regulon transcriptional regulator MetJ	-
LGL97_RS09355 (LGL97_09355)	2095435..2096595	+	1161	WP_039281157.1	cystathionine gamma-synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6560.69 Da
• Isoelectric Point: 10.7838

>T219087 WP_010281507.1 NZ_CP084655:c2091834-2091655 [Pectobacterium brasiliense]
MDSRTLIAEIKADGWELIRVNGSHHHFMHPSKPGLVTIPHPKKDLPIGTVKSIRKQAGI

Nucleotide

• Length: 180 bp

>T219087 NZ_CP116176:c3801381-3800974 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 14492.36 Da
• Isoelectric Point: 4.3287

>AT219087 WP_225583654.1 NZ_CP084655:c2091627-2091217 [Pectobacterium brasiliense]
MFYPIAIEAGDDTHAYGVTVPDLPGCFSAGDTLDDAIANAKDAITGHIELLIEMGQDIPTVSSVGQLAKSAEYAGYTWAV
VDIDVTRLMGGSEKINVTLPKSLIDRIDRCVASNPEFKSRSGFLAQAALERISSSR

Nucleotide

• Length: 411 bp

>AT219087 NZ_CP116176:c3801628-3801362 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A7W2I1S0