Detailed information of TA system TA219085

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 2035672..2036423
• Replicon: chromosome
• Accession: NZ_CP084655
• Organism: Pectobacterium brasiliense strain SR10

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: LGL97_RS09070
• Protein ID: WP_225583689.1
• Coordinates: 2035941..2036423 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A0M2EWB4
• Locus tag: LGL97_RS09065
• Protein ID: WP_039317395.1
• Coordinates: 2035672..2035950 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGL97_RS09040 (LGL97_09040)	2031182..2031838	-	657	WP_010681379.1	aldolase	-
LGL97_RS09045 (LGL97_09045)	2031835..2033109	-	1275	WP_225583693.1	four-carbon acid sugar kinase family protein	-
LGL97_RS09050 (LGL97_09050)	2033106..2034020	-	915	WP_039320187.1	NAD(P)-dependent oxidoreductase	-
LGL97_RS09055 (LGL97_09055)	2034359..2035117	+	759	WP_010281613.1	DNA-binding transcriptional repressor YgbI	-
LGL97_RS09060 (LGL97_09060)	2035281..2035556	+	276	WP_225583691.1	hypothetical protein	-
LGL97_RS09065 (LGL97_09065)	2035672..2035950	+	279	WP_039317395.1	DUF1778 domain-containing protein	Antitoxin
LGL97_RS09070 (LGL97_09070)	2035941..2036423	+	483	WP_225583689.1	GNAT family N-acetyltransferase	Toxin
LGL97_RS09075 (LGL97_09075)	2036556..2037890	+	1335	WP_225583687.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
LGL97_RS09080 (LGL97_09080)	2037963..2038850	+	888	WP_010281599.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
LGL97_RS09085 (LGL97_09085)	2038876..2039721	+	846	WP_010281598.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
LGL97_RS09090 (LGL97_09090)	2039747..2040820	+	1074	WP_010281596.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (39-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-92)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17495.28 Da
• Isoelectric Point: 9.0439

>T219085 WP_225583689.1 NZ_CP084655:2035941-2036423 [Pectobacterium brasiliense]
MGITAPEPLSSSHNLAEFCSQEPSLNEWLKKKALKNHSAGISRVYVVCAEGTHQVIGYYCLSTGSVQRNTAPGAYRRNAP
ESLPVIVLGRLAIDQAYSGKGLGVALLKDAIFRTENIALQVGVRALVVHALDENVRNFYLKFAFDPSPVQPLMLLYPIKI

Nucleotide

• Length: 483 bp

>T219085 NZ_CP116176:c3358001-3357603 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
TCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10667.12 Da
• Isoelectric Point: 4.3611

>AT219085 WP_039317395.1 NZ_CP084655:2035672-2035950 [Pectobacterium brasiliense]
MKPETKEAPINIRAKASQRDLIDMAANLVSKSRTDFMLEAACREAQDILLDQRLFILDNEQFDEFLAELDAPITEERQAR
INDLMSRKSPWE

Nucleotide

• Length: 279 bp

>AT219085 NZ_CP116176:c3358228-3358001 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0M2EWB4