Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PP_4151-4152/excise(antitoxin)
Location 726224..727260 Replicon chromosome
Accession NZ_CP084655
Organism Pectobacterium brasiliense strain SR10

Toxin (Protein)


Gene name PP_4152 Uniprot ID A0A0M2F6X0
Locus tag LGL97_RS03170 Protein ID WP_039312472.1
Coordinates 726224..726799 (-) Length 192 a.a.

Antitoxin (Protein)


Gene name PP_4151 Uniprot ID A0A0M2F6M1
Locus tag LGL97_RS03175 Protein ID WP_039312474.1
Coordinates 726799..727260 (-) Length 154 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGL97_RS03150 (LGL97_03150) 724087..724482 - 396 WP_010286800.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
LGL97_RS03155 (LGL97_03155) 724469..724924 - 456 WP_225584587.1 YgdB family protein -
LGL97_RS03160 (LGL97_03160) 724921..725547 - 627 WP_225584586.1 prepilin peptidase-dependent protein -
LGL97_RS03165 (LGL97_03165) 725541..726044 - 504 WP_225584585.1 prepilin peptidase-dependent protein -
LGL97_RS03170 (LGL97_03170) 726224..726799 - 576 WP_039312472.1 PIN domain-containing protein Toxin
LGL97_RS03175 (LGL97_03175) 726799..727260 - 462 WP_039312474.1 helix-turn-helix domain-containing protein Antitoxin
LGL97_RS03180 (LGL97_03180) 727586..728380 - 795 WP_039312476.1 thymidylate synthase -
LGL97_RS03185 (LGL97_03185) 728417..729283 - 867 WP_039313946.1 prolipoprotein diacylglyceryl transferase -
LGL97_RS03190 (LGL97_03190) 729510..731756 - 2247 WP_052902583.1 phosphoenolpyruvate--protein phosphotransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(8-115)

Antitoxin

SinI-like, DNA-binding domain

(84-131)


Sequences

Toxin        


Download         Length: 192 a.a.        Molecular weight: 21449.86 Da        Isoelectric Point: 4.7154

>T219077 WP_039312472.1 NZ_CP084655:c726799-726224 [Pectobacterium brasiliense]
MNHSPYPVVLDACVIYPSLLRDLLMHLGLTGLYQPKWTALIQDEWQRNLLLNNADVTMDKLRNIEHLMNKALPDANVTGF
EQLIPALVLPDADDRHVLAAAIRANAEVIVTANLKDFPATALSEFAVEAMHPDEFIADLFDLNSALVLKAVRTSRTAFRK
PPFDVDEYLDCLLRQGLPMTVKELDAYRALI

Download         Length: 576 bp

>T219077 NZ_CP116176:c1181529-1181311 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 154 a.a.        Molecular weight: 17271.82 Da        Isoelectric Point: 6.2253

>AT219077 WP_039312474.1 NZ_CP084655:c727260-726799 [Pectobacterium brasiliense]
MKNTPLNSLSLPAPKEMEAAIRGQRQLATYLSTKLETQEISIQDVHDIAHRIELPTSALTLLMSILGELAAGNAVQIVPV
QAELTTQEAANILNVSRPHMVKLLEEGHLPFHKTGRHRRVLFADLMQYKEQRENESMQAMQELTDRSQELGFY

Download         Length: 462 bp

>AT219077 NZ_CP116176:c1181929-1181555 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M2F6X0

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2F6M1

References