Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 522387..523012 Replicon chromosome
Accession NZ_CP084655
Organism Pectobacterium brasiliense strain SR10

Toxin (Protein)


Gene name Hha Uniprot ID C6DB72
Locus tag LGL97_RS02345 Protein ID WP_005976087.1
Coordinates 522809..523012 (+) Length 68 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID Q6D809
Locus tag LGL97_RS02340 Protein ID WP_010282247.1
Coordinates 522387..522755 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGL97_RS02325 (LGL97_02325) 518019..521147 + 3129 WP_039312320.1 efflux RND transporter permease subunit -
LGL97_RS02330 (LGL97_02330) 521380..521628 + 249 WP_039274617.1 type B 50S ribosomal protein L31 -
LGL97_RS02335 (LGL97_02335) 521646..521789 + 144 WP_005976091.1 type B 50S ribosomal protein L36 -
LGL97_RS02340 (LGL97_02340) 522387..522755 + 369 WP_010282247.1 Hha toxicity modulator TomB Antitoxin
LGL97_RS02345 (LGL97_02345) 522809..523012 + 204 WP_005976087.1 HHA domain-containing protein Toxin
LGL97_RS02355 (LGL97_02355) 523754..524074 + 321 WP_010282239.1 MGMT family protein -
LGL97_RS02360 (LGL97_02360) 524094..524675 - 582 WP_010282236.1 YbaY family lipoprotein -
LGL97_RS02365 (LGL97_02365) 524888..525751 + 864 WP_039312322.1 acyl-CoA thioesterase II -
LGL97_RS02370 (LGL97_02370) 525891..527177 - 1287 WP_010282229.1 ammonium transporter AmtB -
LGL97_RS02375 (LGL97_02375) 527217..527555 - 339 WP_002208627.1 P-II family nitrogen regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(8-64)

Antitoxin

Biofilm formation regulator YbaJ

(1-118)


Sequences

Toxin        


Download         Length: 68 a.a.        Molecular weight: 8145.52 Da        Isoelectric Point: 8.9008

>T219076 WP_005976087.1 NZ_CP084655:522809-523012 [Pectobacterium brasiliense]
MKKIDYLMRLRKCTTIDTLERVIEKNKYELSNDELEMFFSAADHRLAELTMNKLYDKVPTAVWRYVR

Download         Length: 204 bp

>T219076 NZ_CP116176:c1147558-1147016 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 14046.80 Da        Isoelectric Point: 4.4787

>AT219076 WP_010282247.1 NZ_CP084655:522387-522755 [Pectobacterium brasiliense]
MDEYTPKHYDIAQLRFLCENLCDESIATLGDSSHGWVNDPTSAINLQLNELIEHIATFILTFKIKYPNESELSEQVEKYL
DDTYVLFSNYGINDAELRRWQKSKAKLFGMFSGENICTPAKT

Download         Length: 369 bp

>AT219076 NZ_CP116176:c1147853-1147542 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D7Z3F0

Antitoxin

Source ID Structure
AlphaFold DB A0A086EI31

References