Detailed information of TA system TA021907

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1660372..1661141
• Replicon: chromosome
• Accession: NC_008781
• Organism: Polaromonas naphthalenivorans CJ2

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: A1VMK7
• Locus tag: PNAP_RS07845
• Protein ID: WP_011800972.1
• Coordinates: 1660372..1660860 (-)
• Length: 163 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: PNAP_RS07850
• Protein ID: WP_011800973.1
• Coordinates: 1660857..1661141 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PNAP_RS07820	1655705..1656028	-	324	WP_011800967.1	iron-sulfur cluster assembly protein IscA	-
PNAP_RS07825	1656040..1656417	-	378	WP_011800968.1	iron-sulfur cluster insertion protein ErpA	-
PNAP_RS07830	1656469..1656921	-	453	WP_011800969.1	Fe-S cluster assembly scaffold IscU	-
PNAP_RS07835	1656985..1658229	-	1245	WP_011800970.1	IscS subfamily cysteine desulfurase	-
PNAP_RS07840	1658567..1660276	+	1710	WP_011800971.1	nif-specific transcriptional activator NifA	-
PNAP_RS07845	1660372..1660860	-	489	WP_011800972.1	GNAT family N-acetyltransferase	Toxin
PNAP_RS07850	1660857..1661141	-	285	WP_011800973.1	DUF1778 domain-containing protein	Antitoxin
PNAP_RS07855	1661636..1663231	+	1596	WP_011800975.1	nitrogenase cofactor biosynthesis protein NifB	-
PNAP_RS07860	1663266..1663460	+	195	WP_011800976.1	4Fe-4S binding protein	-
PNAP_RS07865	1663487..1663897	+	411	WP_011800977.1	iron-sulfur cluster assembly accessory protein	-
PNAP_RS07870	1663924..1664493	+	570	WP_011800978.1	hypothetical protein	-
PNAP_RS07875	1664514..1665329	+	816	WP_157040390.1	hypothetical protein	-
PNAP_RS07880	1665322..1665684	+	363	WP_011800980.1	nitrogen fixation protein NifZ	-
PNAP_RS07885	1665681..1665992	+	312	WP_011800981.1	nitrogen fixation protein NifZ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (44-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (4-92)

Sequences

Toxin

Protein

• Length: 163 a.a.
• Molecular weight: 17488.15 Da
• Isoelectric Point: 8.2775

>T21907 WP_011800972.1 NC_008781:c1660860-1660372 [Polaromonas naphthalenivorans CJ2]
MNFSAPAPLAAHHITEGFACGADSLDNWLKRRALKNQVQGASRTYVVCTENRVVAYYALASGALLGTQATGRLRRNMPDP
IPVVVLGRLAVDSTLRGQGFGRALVRDAGMRVLQAADAIGIRGMTVQALSDEARVFYEHLGFESSPMDPQLLMITLADLK
DC

Nucleotide

• Length: 489 bp

>T21907 NC_008781:c1660860-1660372 [Polaromonas naphthalenivorans CJ2]
ATGAATTTTTCGGCTCCTGCGCCCCTTGCGGCTCATCACATCACCGAGGGCTTTGCTTGTGGGGCGGACAGCCTGGACAA
CTGGCTGAAACGCCGCGCCCTGAAAAATCAGGTTCAGGGCGCCTCACGGACTTACGTGGTGTGCACTGAAAACCGCGTCG
TCGCGTATTACGCACTGGCCTCCGGTGCCTTGCTGGGCACGCAAGCCACGGGGCGCTTACGCCGAAACATGCCAGACCCC
ATTCCGGTGGTGGTACTGGGGCGTTTGGCCGTTGACAGTACCCTTCGCGGTCAGGGCTTTGGCCGGGCTCTGGTTCGGGA
TGCCGGAATGCGTGTTTTGCAGGCCGCGGATGCCATCGGCATTCGCGGCATGACCGTGCAGGCTTTATCCGATGAGGCCA
GGGTTTTTTACGAACACCTTGGGTTTGAGTCCTCCCCCATGGATCCACAACTGTTGATGATCACGCTGGCCGACCTGAAA
GACTGTTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10686.23 Da
• Isoelectric Point: 9.9171

>AT21907 WP_011800973.1 NC_008781:c1661141-1660857 [Polaromonas naphthalenivorans CJ2]
MPAATEVKRETLNIRIKPEERNLIDRAAKTRGKNRTDFILDAARLAAEDALLDQVAISVSPQAYEQFLARLDMPPQANER
LRKTMQTPAPWERG

Nucleotide

• Length: 285 bp

>AT21907 NC_008781:c1661141-1660857 [Polaromonas naphthalenivorans CJ2]
ATGCCCGCAGCCACTGAAGTCAAACGCGAAACCCTCAACATCCGCATCAAACCTGAAGAGCGCAATCTGATTGACCGCGC
CGCCAAAACGCGCGGTAAAAACCGCACCGACTTCATTCTGGATGCCGCGCGTCTGGCGGCGGAAGACGCTTTGCTGGATC
AGGTCGCCATCTCTGTCAGCCCGCAAGCGTATGAGCAATTTCTGGCCCGCCTGGACATGCCGCCTCAAGCCAACGAGCGC
CTGCGCAAAACCATGCAGACACCCGCGCCTTGGGAGCGTGGATGA

Structures

Toxin

• AlphaFold DB: A1VMK7