Detailed information of TA system TA219064

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 4134085..4134610
• Replicon: chromosome
• Accession: NZ_CP084654
• Organism: Pectobacterium versatile strain SR12

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LGL95_RS18425
• Protein ID: WP_039539560.1
• Coordinates: 4134326..4134610 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C6D956
• Locus tag: LGL95_RS18420
• Protein ID: WP_005973616.1
• Coordinates: 4134085..4134336 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGL95_RS18400 (LGL95_18400)	4132492..4132821	-	330	WP_223193302.1	DUF1493 family protein	-
LGL95_RS18405 (LGL95_18405)	4132821..4133291	-	471	WP_103861352.1	hypothetical protein	-
LGL95_RS18410 (LGL95_18410)	4133421..4133672	+	252	WP_105879157.1	prevent-host-death protein	-
LGL95_RS18415 (LGL95_18415)	4133662..4133913	+	252	WP_226305901.1	hypothetical protein	-
LGL95_RS18420 (LGL95_18420)	4134085..4134336	+	252	WP_005973616.1	prevent-host-death protein	Antitoxin
LGL95_RS18425 (LGL95_18425)	4134326..4134610	+	285	WP_039539560.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LGL95_RS18430 (LGL95_18430)	4134876..4135052	+	177	WP_158256803.1	hypothetical protein	-
LGL95_RS18435 (LGL95_18435)	4135270..4135848	+	579	WP_130621939.1	hypothetical protein	-
LGL95_RS18440 (LGL95_18440)	4136216..4136998	+	783	WP_226305902.1	transporter substrate-binding domain-containing protein	-
LGL95_RS18445 (LGL95_18445)	4137254..4137880	+	627	WP_010295795.1	LysE family transporter	-
LGL95_RS18450 (LGL95_18450)	4137926..4138408	-	483	WP_029367525.1	GNAT family N-acetyltransferase	-
LGL95_RS18455 (LGL95_18455)	4138658..4139083	-	426	WP_226305903.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4127403..4135848	8445

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11034.00 Da
• Isoelectric Point: 10.1716

>T219064 WP_039539560.1 NZ_CP084654:4134326-4134610 [Pectobacterium versatile]
MSYKLEFEEHALKEFKKLGAPVREQFTKKLKEVLQHPHVPANRLHGMADCYKIKLRSAGYRLVYQVLEHEIVVLVLAVGK
RERSEVYKTAKDRL

Nucleotide

• Length: 285 bp

>T219064 NZ_CP116169:3460876-3461094 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9079.35 Da
• Isoelectric Point: 5.0807

>AT219064 WP_005973616.1 NZ_CP084654:4134085-4134336 [Pectobacterium versatile]
MSVQPILANAAVSISDFKKSPNAALKEANGEPVAVLTNGRISGYYVSPETWEALADYQEDIELAKIARSRMKGKRVKVDL
DEL

Nucleotide

• Length: 252 bp

>AT219064 NZ_CP116169:3460476-3460850 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A0G4JQP6