Detailed information of TA system TA219057

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 3001380..3001906
• Replicon: chromosome
• Accession: NZ_CP084654
• Organism: Pectobacterium versatile strain SR12

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LGL95_RS13295
• Protein ID: WP_107333022.1
• Coordinates: 3001619..3001906 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: LGL95_RS13290
• Protein ID: WP_107333021.1
• Coordinates: 3001380..3001619 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGL95_RS13275 (LGL95_13275)	2997117..2998367	+	1251	WP_226305749.1	integrase domain-containing protein	-
LGL95_RS13280 (LGL95_13280)	2998717..3000159	+	1443	WP_043135453.1	IS1182 family transposase	-
LGL95_RS13285 (LGL95_13285)	3000456..3001073	+	618	WP_019083811.1	LysE family transporter	-
LGL95_RS13290 (LGL95_13290)	3001380..3001619	+	240	WP_107333021.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
LGL95_RS13295 (LGL95_13295)	3001619..3001906	+	288	WP_107333022.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LGL95_RS13300 (LGL95_13300)	3001924..3002283	+	360	WP_107333023.1	hypothetical protein	-
LGL95_RS13305 (LGL95_13305)	3002482..3002841	-	360	WP_039475580.1	ankyrin repeat domain-containing protein	-
LGL95_RS13310 (LGL95_13310)	3003226..3003696	-	471	WP_219606901.1	GyrI-like domain-containing protein	-
LGL95_RS13315 (LGL95_13315)	3003838..3004560	-	723	WP_107333024.1	alpha/beta fold hydrolase	-
LGL95_RS13320 (LGL95_13320)	3004804..3005133	-	330	WP_010294841.1	DHCW motif cupin fold protein	-
LGL95_RS13325 (LGL95_13325)	3005200..3005736	-	537	WP_107333025.1	rhodanese family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-84)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (3-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11302.14 Da
• Isoelectric Point: 10.1960

>T219057 WP_107333022.1 NZ_CP084654:3001619-3001906 [Pectobacterium versatile]
MSYFLDFDERALKEWRKLGETVRNQFKKKLTDILDAPRIEANKLRGMPDCYKIKLRSAGYRLVYQVQDEKVVVFVVSVGK
RERSEAYDDAIKRLR

Nucleotide

• Length: 288 bp

>T219057 NZ_CP116169:1819953-1820207 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9037.34 Da
• Isoelectric Point: 4.5112

>AT219057 WP_107333021.1 NZ_CP084654:3001380-3001619 [Pectobacterium versatile]
MGSINLRIDDDLKTRSYAALEKLGVTPSEALRLTLEYIAENERLPFKQALLSDEDAELVEIVRERLRNPKPVRVTLDDL

Nucleotide

• Length: 240 bp

>AT219057 NZ_CP116169:1819705-1819956 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA