Detailed information of TA system TA219050

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 151619..152325
• Replicon: chromosome
• Accession: NZ_CP084654
• Organism: Pectobacterium versatile strain SR12

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LGL95_RS00600
• Protein ID: WP_226306090.1
• Coordinates: 152068..152325 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: LGL95_RS00595
• Protein ID: WP_010303241.1
• Coordinates: 151619..151948 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGL95_RS00570 (LGL95_00570)	146841..147623	-	783	WP_116166171.1	hydroxypyruvate isomerase family protein	-
LGL95_RS00575 (LGL95_00575)	147657..148313	-	657	WP_116166170.1	aldolase	-
LGL95_RS00580 (LGL95_00580)	148310..149584	-	1275	WP_226306089.1	four-carbon acid sugar kinase family protein	-
LGL95_RS00585 (LGL95_00585)	149581..150495	-	915	WP_103862322.1	NAD(P)-dependent oxidoreductase	-
LGL95_RS00590 (LGL95_00590)	150834..151595	+	762	WP_109225580.1	DNA-binding transcriptional repressor YgbI	-
LGL95_RS00595 (LGL95_00595)	151619..151948	-	330	WP_010303241.1	helix-turn-helix domain-containing protein	Antitoxin
LGL95_RS00600 (LGL95_00600)	152068..152325	-	258	WP_226306090.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LGL95_RS00605 (LGL95_00605)	152536..152706	+	171	Protein_120	DeoR family transcriptional regulator	-
LGL95_RS00610 (LGL95_00610)	152823..153518	+	696	WP_180782328.1	hypothetical protein	-
LGL95_RS00615 (LGL95_00615)	153484..153936	+	453	WP_226306091.1	hypothetical protein	-
LGL95_RS00620 (LGL95_00620)	154047..154325	+	279	WP_226306467.1	DUF1778 domain-containing protein	-
LGL95_RS00625 (LGL95_00625)	154316..154795	+	480	Protein_124	GNAT family N-acetyltransferase	-
LGL95_RS00630 (LGL95_00630)	154928..156262	+	1335	WP_010303220.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
LGL95_RS00635 (LGL95_00635)	156334..157221	+	888	WP_226306092.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (3-80)

Antitoxin

• Cro/C1-type helix-turn-helix domain (56-96)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9426.96 Da
• Isoelectric Point: 10.4787

>T219050 WP_226306090.1 NZ_CP084654:c152325-152068 [Pectobacterium versatile]
VAVYTTKLFDKKLKSEILSDSYLCEIAHEVMNGRFEGDLGGGVIKKRIALNAGKSAGARTIIFFKLGSNLFFADGWKKKS
GQERR

Nucleotide

• Length: 258 bp

>T219050 NZ_CP116169:265338-265568 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12370.16 Da
• Isoelectric Point: 9.9987

>AT219050 WP_010303241.1 NZ_CP084654:c151948-151619 [Pectobacterium versatile]
MAAKMKELQEMAKSLHEHGGITDTTMERINNRVAAREFRERIEQAHAMSGAEIQNMRSRYKMSQSSLAYALNMSVESVSK
WERNEKKPSGAALRLLNIINEKGLDIFAS

Nucleotide

• Length: 330 bp

>AT219050 NZ_CP116169:265565-265924 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA