Detailed information of TA system TA219022

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 974555..975178
• Replicon: chromosome
• Accession: NZ_CP084650
• Organism: Yersinia ruckeri strain 17Y0153

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: -
• Locus tag: LGL87_RS04425
• Protein ID: WP_049687933.1
• Coordinates: 974555..974758 (-)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: A0A085U5C5
• Locus tag: LGL87_RS04430
• Protein ID: WP_004718732.1
• Coordinates: 974810..975178 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGL87_RS04400 (LGL87_04405)	970410..970748	+	339	WP_007886947.1	P-II family nitrogen regulator	-
LGL87_RS04405 (LGL87_04410)	970787..972076	+	1290	WP_004718723.1	ammonium transporter AmtB	-
LGL87_RS04410 (LGL87_04415)	972191..973054	-	864	WP_004718726.1	acyl-CoA thioesterase II	-
LGL87_RS04415 (LGL87_04420)	973432..973746	-	315	WP_004718728.1	MGMT family protein	-
LGL87_RS04425 (LGL87_04430)	974555..974758	-	204	WP_049687933.1	expression modulating protein YmoA	Toxin
LGL87_RS04430 (LGL87_04435)	974810..975178	-	369	WP_004718732.1	Hha toxicity modulator TomB	Antitoxin
LGL87_RS04435 (LGL87_04440)	976227..976388	+	162	WP_004718735.1	hypothetical protein	-
LGL87_RS04440 (LGL87_04445)	976507..976650	-	144	WP_004718738.1	type B 50S ribosomal protein L36	-
LGL87_RS04445 (LGL87_04450)	976670..976924	-	255	WP_038243728.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8019.24 Da
• Isoelectric Point: 6.4573

>T219022 WP_049687933.1 NZ_CP084650:c974758-974555 [Yersinia ruckeri]
MTKTDYLIRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPATVWQHVK

Nucleotide

• Length: 204 bp

>T219022 NZ_CP116159:3733887-3734165 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14277.08 Da
• Isoelectric Point: 4.4225

>AT219022 WP_004718732.1 NZ_CP084650:c975178-974810 [Yersinia ruckeri]
MDEYSPKRHDIAQLKFLCENLYDEGIATLGDSHHGWVNDPTSAVNLQLNDLIEHIASFVMSFKIKYADEIDLCELVEEYL
DDTYTLFSSYGINDPDLRRWQKTKDRLFRLFSGEYLCALMKN

Nucleotide

• Length: 369 bp

>AT219022 NZ_CP116159:3733624-3733884 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Antitoxin

• AlphaFold DB: A0A085U5C5