Detailed information of TA system TA219009

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1005519..1006142
• Replicon: chromosome
• Accession: NZ_CP084648
• Organism: Yersinia ruckeri strain 17Y0155

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: -
• Locus tag: LGL92_RS04560
• Protein ID: WP_049687933.1
• Coordinates: 1005519..1005722 (-)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: A0A085U5C5
• Locus tag: LGL92_RS04565
• Protein ID: WP_004718732.1
• Coordinates: 1005774..1006142 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGL92_RS04535 (LGL92_04550)	1001375..1001713	+	339	WP_007886947.1	P-II family nitrogen regulator	-
LGL92_RS04540 (LGL92_04555)	1001752..1003040	+	1289	Protein_879	ammonium transporter AmtB	-
LGL92_RS04545 (LGL92_04560)	1003155..1004018	-	864	WP_004718726.1	acyl-CoA thioesterase II	-
LGL92_RS04550 (LGL92_04565)	1004396..1004710	-	315	WP_004718728.1	MGMT family protein	-
LGL92_RS04560 (LGL92_04575)	1005519..1005722	-	204	WP_049687933.1	expression modulating protein YmoA	Toxin
LGL92_RS04565 (LGL92_04580)	1005774..1006142	-	369	WP_004718732.1	Hha toxicity modulator TomB	Antitoxin
LGL92_RS04570 (LGL92_04585)	1007191..1007352	+	162	WP_004718735.1	hypothetical protein	-
LGL92_RS04575 (LGL92_04590)	1007471..1007614	-	144	WP_004718738.1	type B 50S ribosomal protein L36	-
LGL92_RS04580 (LGL92_04595)	1007634..1007888	-	255	WP_038243728.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8019.24 Da
• Isoelectric Point: 6.4573

>T219009 WP_049687933.1 NZ_CP084648:c1005722-1005519 [Yersinia ruckeri]
MTKTDYLIRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPATVWQHVK

Nucleotide

• Length: 204 bp

>T219009 NZ_CP116159:c796368-795994 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14277.08 Da
• Isoelectric Point: 4.4225

>AT219009 WP_004718732.1 NZ_CP084648:c1006142-1005774 [Yersinia ruckeri]
MDEYSPKRHDIAQLKFLCENLYDEGIATLGDSHHGWVNDPTSAVNLQLNDLIEHIASFVMSFKIKYADEIDLCELVEEYL
DDTYTLFSSYGINDPDLRRWQKTKDRLFRLFSGEYLCALMKN

Nucleotide

• Length: 369 bp

>AT219009 NZ_CP116159:c796826-796458 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A085U5C5