Detailed information of TA system TA218992

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 453231..453765
• Replicon: chromosome
• Accession: NZ_CP084647
• Organism: Yersinia ruckeri strain 17Y0157

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A0T9LYG3
• Locus tag: LGL89_RS02040
• Protein ID: WP_038275268.1
• Coordinates: 453475..453765 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A1JJ37
• Locus tag: LGL89_RS02035
• Protein ID: WP_005175264.1
• Coordinates: 453231..453485 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGL89_RS02010 (LGL89_02020)	448333..449589	+	1257	Protein_388	integrase arm-type DNA-binding domain-containing protein	-
LGL89_RS02015 (LGL89_02025)	449661..449966	+	306	WP_234054908.1	hypothetical protein	-
LGL89_RS02020 (LGL89_02030)	450017..451273	-	1257	WP_234054909.1	hypothetical protein	-
LGL89_RS02030 (LGL89_02040)	452683..453057	+	375	Protein_392	tyrosine-type recombinase/integrase	-
LGL89_RS02035 (LGL89_02045)	453231..453485	+	255	WP_005175264.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
LGL89_RS02040 (LGL89_02050)	453475..453765	+	291	WP_038275268.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LGL89_RS02045 (LGL89_02055)	454029..454418	+	390	WP_096823270.1	restriction endonuclease subunit S	-
LGL89_RS02050 (LGL89_02060)	454542..455117	+	576	WP_234049239.1	DarT ssDNA thymidine ADP-ribosyltransferase family protein	-
LGL89_RS02055 (LGL89_02065)	455120..455776	+	657	WP_096823271.1	DUF4433 domain-containing protein	-
LGL89_RS02060 (LGL89_02070)	455773..456840	+	1068	WP_038245756.1	macro domain-containing protein	-
LGL89_RS02065 (LGL89_02075)	456861..457127	+	267	Protein_399	hypothetical protein	-
LGL89_RS02070 (LGL89_02080)	457146..457544	-	399	Protein_400	integrase core domain-containing protein	-
LGL89_RS02075 (LGL89_02085)	457543..457839	+	297	Protein_401	integrase core domain-containing protein	-
LGL89_RS02080 (LGL89_02090)	457868..457973	-	106	Protein_402	transposase	-
LGL89_RS02085 (LGL89_02095)	457987..458115	-	129	Protein_403	helix-turn-helix domain-containing protein	-
LGL89_RS02090 (LGL89_02100)	458265..458495	+	231	Protein_404	DUF4942 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	440574..456840	16266
	-	flank	IS/Tn	-	-	457591..457839	248

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-75)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11494.42 Da
• Isoelectric Point: 9.9816

>T218992 WP_038275268.1 NZ_CP084647:453475-453765 [Yersinia ruckeri]
MTFNIDFDERALKEWHKLDKTIREQFKKKLRKLQENPYIESARLHGDLAGCFKIKLRASGFRLIYQVIDEEIVIWIVAVG
KREDEKAYDIAKKRIQ

Nucleotide

• Length: 291 bp

>T218992 NZ_CP116152:2579588-2579695 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9680.07 Da
• Isoelectric Point: 4.8283

>AT218992 WP_005175264.1 NZ_CP084647:453231-453485 [Yersinia ruckeri]
MSTIQIRVDDELKKSAYQAFEKLNLSPSDALRLFLRYVAENEKLPFSEVSVMVSNQDEDADILAVVRERLKNPAKRIRVN
IDDL

Nucleotide

• Length: 255 bp

>AT218992 NZ_CP116152:c2579541-2579475 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Structures

Toxin

• AlphaFold DB: A0A0T9LYG3

Antitoxin

• AlphaFold DB: A1JJ37