Detailed information of TA system TA218989

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 45266..45848
• Replicon: plasmid p17Y0159.1
• Accession: NZ_CP084644
• Organism: Yersinia ruckeri strain 17Y0159

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: LGL91_RS17810
• Protein ID: WP_234057223.1
• Coordinates: 45266..45562 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: LGL91_RS17815
• Protein ID: WP_234057224.1
• Coordinates: 45564..45848 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGL91_RS17775 (LGL91_17825)	40449..41003	+	555	Protein_56	ISAs1 family transposase	-
LGL91_RS17780 (LGL91_17830)	41046..42246	+	1201	Protein_57	IS3 family transposase	-
LGL91_RS17785 (LGL91_17835)	42486..43037	-	552	WP_265099274.1	IS110 family transposase	-
LGL91_RS17795 (LGL91_17845)	44278..44757	-	480	Protein_60	IS110 family transposase	-
LGL91_RS17800 (LGL91_17850)	44830..44931	-	102	Protein_61	IS6 family transposase	-
LGL91_RS17805 (LGL91_17855)	44973..45194	+	222	WP_234057222.1	ProQ/FINO family protein	-
LGL91_RS17810 (LGL91_17860)	45266..45562	+	297	WP_234057223.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LGL91_RS17815 (LGL91_17865)	45564..45848	+	285	WP_234057224.1	putative addiction module antidote protein	Antitoxin
LGL91_RS17820 (LGL91_17870)	45903..46592	-	690	WP_234057225.1	IS6 family transposase	-
LGL91_RS17825 (LGL91_17875)	47312..49066	+	1755	WP_234057226.1	ShlB/FhaC/HecB family hemolysin secretion/activation protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..85059	85059

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Uncharacterized protein HI1420 (5-90)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11334.30 Da
• Isoelectric Point: 10.2781

>T218989 WP_234057223.1 NZ_CP084644:45266-45562 [Yersinia ruckeri]
MIEIRRTLEVEKWLKSLKDRMAKAKILMRIERMEEGHFGDVEAIGNGLSELRIHQGKGYRLYFGNKNNQVILLLCGGDKS
TQQEDIKKAKLLATKWGF

Nucleotide

• Length: 297 bp

>T218989 NZ_CP116152:1965831-1965934 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10502.87 Da
• Isoelectric Point: 4.6794

>AT218989 WP_234057224.1 NZ_CP084644:45564-45848 [Yersinia ruckeri]
MNNELKKFDVVEFLDDEEDIQEYLNAAIEEDDAKYLFKALGNIARARNMSQLSKEVGMSREGIYKALSGEGNPSFQTVSK
ITKALGLKLHFTGA

Nucleotide

• Length: 285 bp

>AT218989 NZ_CP116152:c1965972-1965701 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT