Detailed information of TA system TA218988

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 3779..4406
• Replicon: plasmid p17Y0159.1
• Accession: NZ_CP084644
• Organism: Yersinia ruckeri strain 17Y0159

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: LGL91_RS17535
• Protein ID: WP_234057258.1
• Coordinates: 4227..4406 (-)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LGL91_RS17530
• Protein ID: WP_234057257.1
• Coordinates: 3779..4198 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGL91_RS17500 (LGL91_17540)	4..837	+	834	WP_234057290.1	MurR/RpiR family transcriptional regulator	-
LGL91_RS17505 (LGL91_17545)	974..1306	+	333	Protein_1	ISKra4 family transposase	-
LGL91_RS17510 (LGL91_17550)	1345..2031	+	687	Protein_2	IS66 family transposase	-
LGL91_RS17515 (LGL91_17555)	2058..2852	-	795	WP_234057254.1	ISAs1 family transposase	-
LGL91_RS17520 (LGL91_17560)	2940..3158	-	219	WP_234057255.1	hypothetical protein	-
LGL91_RS17525 (LGL91_17565)	3155..3637	-	483	WP_234057256.1	hypothetical protein	-
LGL91_RS17530 (LGL91_17570)	3779..4198	-	420	WP_234057257.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LGL91_RS17535 (LGL91_17575)	4227..4406	-	180	WP_234057258.1	type II toxin-antitoxin system HicA family toxin	Toxin
LGL91_RS17540 (LGL91_17580)	4635..4892	+	258	WP_234057259.1	ParA family protein	-
LGL91_RS17545 (LGL91_17585)	4887..5576	-	690	Protein_9	IS256 family transposase	-
LGL91_RS17550 (LGL91_17590)	5635..6432	+	798	WP_234057260.1	aconitase family protein	-
LGL91_RS17555 (LGL91_17595)	6443..6946	+	504	WP_234057261.1	3-isopropylmalate dehydratase	-
LGL91_RS17560 (LGL91_17600)	6936..8093	+	1158	WP_234057262.1	PrpF domain-containing protein	-
LGL91_RS17565 (LGL91_17605)	8446..9131	+	686	Protein_13	IS6 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..85059	85059

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6632.74 Da
• Isoelectric Point: 11.1475

>T218988 WP_234057258.1 NZ_CP084644:c4406-4227 [Yersinia ruckeri]
MDSRKLIAEVRADGWELVRVNGSHHHFTHPTKSGLVTIPHPKKDLPIKTVKSIRKQAGI

Nucleotide

• Length: 180 bp

>T218988 NZ_CP116152:939218-939553 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15007.19 Da
• Isoelectric Point: 4.4686

>AT218988 WP_234057257.1 NZ_CP084644:c4198-3779 [Yersinia ruckeri]
MLYPIAIETGDEQHAYGVTVPDLQGCFSAGDTMDEAITNAKEAITGHIELLVEMGANIPAVSPLSVLAKDPEYSGYTWAV
VDIDVTRLLGGSEKINVTLPKSLIDRIDRCVASNPEFKTRSGFLAQVALERIAVMHKCT

Nucleotide

• Length: 420 bp

>AT218988 NZ_CP116152:938970-939218 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA