Detailed information of TA system TA218975

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 465508..467228
• Replicon: chromosome
• Accession: NZ_CP084643
• Organism: Yersinia ruckeri strain 17Y0159

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: LGL91_RS02140
• Protein ID: WP_096823271.1
• Coordinates: 465508..466164 (+)
• Length: 219 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: LGL91_RS02145
• Protein ID: WP_096823272.1
• Coordinates: 466161..467228 (+)
• Length: 356 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGL91_RS02105 (LGL91_02120)	461410..461733	+	324	Protein_398	Arm DNA-binding domain-containing protein	-
LGL91_RS02110 (LGL91_02125)	461751..462898	-	1148	Protein_399	IS3 family transposase	-
LGL91_RS02115 (LGL91_02130)	463071..463445	+	375	Protein_400	tyrosine-type recombinase/integrase	-
LGL91_RS02120 (LGL91_02135)	463619..463873	+	255	WP_234056963.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
LGL91_RS02125 (LGL91_02140)	463863..464153	+	291	WP_038275268.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LGL91_RS02130 (LGL91_02145)	464417..464806	+	390	WP_234056964.1	restriction endonuclease subunit S	-
LGL91_RS02135 (LGL91_02150)	464930..465505	+	576	WP_234049239.1	DarT ssDNA thymidine ADP-ribosyltransferase family protein	-
LGL91_RS02140 (LGL91_02155)	465508..466164	+	657	WP_096823271.1	DUF4433 domain-containing protein	Toxin
LGL91_RS02145 (LGL91_02160)	466161..467228	+	1068	WP_096823272.1	macro domain-containing protein	Antitoxin
LGL91_RS02150 (LGL91_02165)	467249..467515	+	267	Protein_407	hypothetical protein	-
LGL91_RS02155 (LGL91_02170)	467534..467932	-	399	Protein_408	integrase core domain-containing protein	-
LGL91_RS02160 (LGL91_02175)	467931..468227	+	297	Protein_409	integrase core domain-containing protein	-
LGL91_RS02165 (LGL91_02180)	468256..468376	-	121	Protein_410	transposase	-
LGL91_RS02170 (LGL91_02185)	468369..468497	-	129	Protein_411	helix-turn-helix domain-containing protein	-
LGL91_RS02175 (LGL91_02190)	468647..468877	+	231	Protein_412	DUF4942 domain-containing protein	-
LGL91_RS02180 (LGL91_02195)	468952..469347	+	396	WP_038245317.1	DUF6088 family protein	-
LGL91_RS02185 (LGL91_02200)	469340..470290	+	951	WP_234055605.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
LGL91_RS02190 (LGL91_02205)	470694..470819	+	126	Protein_415	antirestriction protein	-
LGL91_RS02195 (LGL91_02210)	470845..471018	+	174	Protein_416	type IV toxin-antitoxin system YeeU family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	461410..467228	5818
	-	flank	IS/Tn	-	-	467979..468227	248

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (17-217)

Antitoxin

• Macro domain (18-129)

Sequences

Toxin

Protein

• Length: 219 a.a.
• Molecular weight: 25214.72 Da
• Isoelectric Point: 6.6007

>T218975 WP_096823271.1 NZ_CP084643:465508-466164 [Yersinia ruckeri]
VAYDYSTSLNPEKALIWRIVHRDNIPWILDNGLHCGNSPVQAEDWINIGNPELIDKRAGHLVPTGMRGTLHDYVPFYFTP
FSPMLMNIRSGRGGIKQRPNEEIVILVSSLRNMVAQNVSFVFTDSHAYYNLANYFTELDSLNQIDWPILQARDFRRDQDD
LAKFERYQAEALIWKHCPIALLGGMICYNDGVRLQLEQWLSQRNLSMPVHARAGWYFS

Nucleotide

• Length: 657 bp

>T218975 NZ_CP116145:3841917-3842837 [Escherichia coli]
ATGGATCTGCGTGATAGCTATGCCAGGCAGGTTAAACTGTTGATGGCCGCTCTGCCGCATGTGGCGAAGGAATCCTGTTT
CGCGTTGAAAGGCGGCACAGCAATCAATTTATTTGTGCAGGATTTCCCCCGTCTATCGGTGGATATCGATCTGGCGTATA
AGAGCTTTGCGGATCGCGATACCGATCTGGCGGCGATCAACGACGCTTTGAAGAGAATTACTGCATCGCTGAACGGCAGA
CCGGGGATCACCGCGATTCGGCAGGAAAATAAAGCGGATGAAAAGCGCATCATTGTGAATACCGCTGATGCAAAGATTAA
GATCGAGGTTAGCCCGGTCTGGCGCGGGCTGTTGTTGCCTCCGGCAGAAATGCCTGTGTGCGAACGTGTGGAAATGGAGT
ATGGCTTCACCACCATGAGTGTCGTCTCGCTGGCGGATCTTTATGGCGGTAAAATATGTGCTGCGCTTGATCGTCAGCAT
CCGCGCGATCTGTTTGATGTGTTGAATATGCTGGGGAGACCGGGCCTGACGCGCGAAATTTTTGACGGTTTTCTTTGTTA
TCTGGCAGGCCATCCCCGTCCCATTGCCGAGTTGCTGGCTTCAAACTGGGATACAGATCGGATTGCCACGTTATACGCTC
AGGAATTTTCGGGGATGACTCAGCAGGAAACGTCGCTGGAATCGCTTCTGTCAGTAACGACTTTGCTGCCGCAGGCGTTG
AAGTCACATTTTACGGATCTGGATCGCGAGTTCTTGCTCAGTTTTAAGCAAAACAGCCCTGACTGGTCGCGGTATCGCTA
TCCTGAAATTCAGCATCTTCCGGCTATTCGCTGGAAGCAACGCAATCTGGCGATGCTTAAAGATAAAAATCCGGCGAAGT
ATGTGGCGGCGGTGAATAAGCTAGAGAGGGTGCTGGAGTAA

Antitoxin

Protein

• Length: 356 a.a.
• Molecular weight: 40847.21 Da
• Isoelectric Point: 6.3136

>AT218975 WP_096823272.1 NZ_CP084643:466161-467228 [Yersinia ruckeri]
MITFTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPVNMKAYALACKHKQVMTGKMFITETGELMGPRWIVNFPTK
QHWRADSRMEWVEDGLQDLRRFLIEEKVQSIAIPPLGAGNGGLNWSDVRMRIESALGDLQDTEILIYEPTEKYQNVAKST
GVKKLTPARALIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQEDVLKLRFEAHYYGPYAPNLNHLLNALDGTYLK
AEKRIPDTQPLDVIWFNDQEKEHINLWLNNEAKEWLPALEQVSQLIDGFESPFGLELLATVDWLLTRGKCQPTLDSVKEG
LHQWPAGERWASRKMKLFDDKNLQFAINRVMEFHC

Nucleotide

• Length: 1068 bp

>AT218975 NZ_CP116145:3841148-3841930 [Escherichia coli]
TTGCCAAACTATCTAAACTGGTTGATGCAAAACACGCTACCCGGTCAGGTGCTGCTCCAGTCATGGCTTACCCGCAATGG
CGTAGATCGTAAGCTTTCTTATCTCTATGTTCAGAATGGCTGGCTCAGGCGCATCGCTCACGGTGTGTACTGCCGGACGG
GCCGTGAGCCGGATTGGGTAGACGCGGTTTACTGCCTTCAAACACAGTGGGAAAAGCCTGTTCGGGTGGCGGGGCTAACC
AGCCTTGCGCTTCAGGGCCATGCGCACTATACCGAACCGGGTAAGCCGCAGGTCTGGCTCGCGCTTCCGGCTTACGTCAA
ACTGCCGGGATGGTTTGCTGCCTTCGAACAATCAGCAACATTTATCACGCTGTATACATCGGGCCTTACCGTTGATGTCT
CGTCATTTACTACGGAACTGAAAATTGGCGACCGGCAGTTAGCAGGCAGTGCACCGGAACTGGCCGCGTATGAGATCGCC
TGCGGCGTTCCTTCGTTGATCTCGTTCGAACATGCCGATGCTCTGTTTCAGGGGGGGAATCTTCTCAGCCCACGCAAATT
GCAGGCACTGCTTCGCGCCAGTCATTCGATTAAAACCAACCGGGTGATGCTTTACCTTGCCCGGCGTAATGGACACCCTT
ATTTTCAGTATCTTGATCAAAGCGGTATAGAAACAGGTACGGGAAAGCGGCAAATCATACCTGGTGGCTGGCTGGAGCCG
GATTATCAAATTACTGTTCCCCGCACATTTAAACCGGAAGGAGTCGAAGATGGATCTGCGTGA