Detailed information of TA system TA218960

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 687145..687814
• Replicon: chromosome
• Accession: NZ_CP084642
• Organism: Yersinia ruckeri strain 17Y0161

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: A0A380QS17
• Locus tag: LGL90_RS03050
• Protein ID: WP_038241020.1
• Coordinates: 687392..687814 (+)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: -
• Locus tag: LGL90_RS03045
• Protein ID: WP_004719196.1
• Coordinates: 687145..687411 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGL90_RS03030 (LGL90_03035)	684482..684738	-	257	Protein_584	HD domain-containing protein	-
LGL90_RS03035 (LGL90_03040)	685055..685720	+	666	WP_175537887.1	hemolysin III family protein	-
LGL90_RS03040 (LGL90_03045)	685859..686851	-	993	WP_038241018.1	tRNA-modifying protein YgfZ	-
LGL90_RS03045 (LGL90_03050)	687145..687411	+	267	WP_004719196.1	FAD assembly factor SdhE	Antitoxin
LGL90_RS03050 (LGL90_03055)	687392..687814	+	423	WP_038241020.1	protein YgfX	Toxin
LGL90_RS03055 (LGL90_03060)	687962..688480	-	519	WP_038241022.1	flavodoxin FldB	-
LGL90_RS03060 (LGL90_03065)	688588..689487	+	900	WP_004719193.1	site-specific tyrosine recombinase XerD	-
LGL90_RS03065 (LGL90_03070)	689519..690235	+	717	WP_004719192.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
LGL90_RS03070 (LGL90_03075)	690242..691975	+	1734	WP_038276198.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (2-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 16451.41 Da
• Isoelectric Point: 11.1478

>T218960 WP_038241020.1 NZ_CP084642:687392-687814 [Yersinia ruckeri]
VAQWRCDLRVSWRTQLFSLLVHGVLVLLTLVVPWPDGFTPLWLILLTLVVFECIRSQKNITSRQGEIRLKAGNLLIWKWQ
EWTLVRPPWITRYGVLLSLQGAGSRRRKRIWLASDSMSEEKWRELCLILRHTFDSAAGRE

Nucleotide

• Length: 423 bp

>T218960 NZ_CP116145:c759238-758987 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10532.09 Da
• Isoelectric Point: 6.7185

>AT218960 WP_004719196.1 NZ_CP084642:687145-687411 [Yersinia ruckeri]
MEIDNKARIHWACRRGMRELDISIMPFFEHEYDTLNDNDKRVFIRLLTSDDPDLFNWLMNHGEPQDSELKRMVKLIQTRN
KARGPVAM

Nucleotide

• Length: 267 bp

>AT218960 NZ_CP116145:c758990-758736 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: A0A380QS17