Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 3137070..3137757 Replicon chromosome
Accession NC_008769
Organism Mycobacterium bovis BCG str. Pasteur 1173P2

Toxin (Protein)


Gene name vapC Uniprot ID A0A7U4FBY8
Locus tag BCG_RS14900 Protein ID WP_011799298.1
Coordinates 3137314..3137757 (+) Length 148 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TFH0
Locus tag BCG_RS14895 Protein ID WP_003414620.1
Coordinates 3137070..3137327 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BCG_RS14875 3132390..3133244 - 855 WP_003899516.1 GNAT family N-acetyltransferase -
BCG_RS14880 3133300..3134463 - 1164 WP_003899517.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
BCG_RS14885 3134480..3135694 - 1215 WP_011799297.1 zinc metalloprotease Rip -
BCG_RS14890 3135702..3136943 - 1242 WP_003414613.1 1-deoxy-D-xylulose-5-phosphate reductoisomerase -
BCG_RS14895 3137070..3137327 + 258 WP_003414620.1 ribbon-helix-helix domain-containing protein Antitoxin
BCG_RS14900 3137314..3137757 + 444 WP_011799298.1 type II toxin-antitoxin system VapC family toxin Toxin
BCG_RS14905 3137837..3138499 + 663 WP_003414630.1 cell surface glycolipoprotein Mpt83 -
BCG_RS14910 3138595..3138786 + 192 WP_003414632.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
BCG_RS14915 3139118..3140866 + 1749 WP_003912869.1 cytochrome c biogenesis protein DipZ -
BCG_RS14920 3140962..3141543 + 582 WP_003414644.1 fasciclin domain-containing protein -
BCG_RS14925 3141643..3141909 + 267 WP_031657293.1 DUF2631 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-142)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 148 a.a.        Molecular weight: 16625.74 Da        Isoelectric Point: 6.4890

>T21896 WP_011799298.1 NC_008769:3137314-3137757 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDTLLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Download         Length: 444 bp

>T21896 NC_008769:3137314-3137757 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACGCGGACTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGAGCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACACCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9013.22 Da        Isoelectric Point: 10.8344

>AT21896 WP_003414620.1 NC_008769:3137070-3137327 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Download         Length: 258 bp

>AT21896 NC_008769:3137070-3137327 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
GTGCGCACGACGATCCGTATCGATGACGAGCTGTACCGCGAGGTGAAAGCAAAGGCCGCTCGTTCCGGGCGTACCGTGGC
CGCGGTTCTTGAAGATGCGGTGCGGCGTGGTCTCAACCCGCCTAAGCCGCAGGCCGCCGGCCGTTATCGAGTCCAGCCGT
CGGGTAAGGGCGGCCTGCGGCCCGGTGTCGATCTATCGTCCAACGCCGCACTTGCCGAAGCGATGAACGACGGCGTGTCG
GTCGATGCTGTGCGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBY8

Antitoxin

Source ID Structure
PDB 7VWO
AlphaFold DB G0TFH0

References