Detailed information of TA system TA218959

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 3276482..3277016
• Replicon: chromosome
• Accession: NZ_CP084641
• Organism: Yersinia ruckeri strain 17Y0163

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A0T9LYG3
• Locus tag: LGL88_RS15065
• Protein ID: WP_038275268.1
• Coordinates: 3276482..3276772 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A1JJ37
• Locus tag: LGL88_RS15070
• Protein ID: WP_005175264.1
• Coordinates: 3276762..3277016 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGL88_RS15015 (LGL88_15050)	3271752..3271982	-	231	Protein_2913	DUF4942 domain-containing protein	-
LGL88_RS15020 (LGL88_15055)	3272132..3272260	+	129	Protein_2914	helix-turn-helix domain-containing protein	-
LGL88_RS15025 (LGL88_15060)	3272274..3272379	+	106	Protein_2915	transposase	-
LGL88_RS15030 (LGL88_15065)	3272408..3272704	-	297	Protein_2916	integrase core domain-containing protein	-
LGL88_RS15035 (LGL88_15070)	3272703..3273101	+	399	Protein_2917	integrase core domain-containing protein	-
LGL88_RS15040 (LGL88_15075)	3273120..3273386	-	267	Protein_2918	hypothetical protein	-
LGL88_RS15045 (LGL88_15080)	3273407..3274474	-	1068	WP_096823272.1	macro domain-containing protein	-
LGL88_RS15050 (LGL88_15085)	3274471..3275127	-	657	WP_096823271.1	DUF4433 domain-containing protein	-
LGL88_RS15055 (LGL88_15090)	3275130..3275705	-	576	WP_234055606.1	DarT ssDNA thymidine ADP-ribosyltransferase family protein	-
LGL88_RS15060 (LGL88_15095)	3275829..3276218	-	390	WP_234055607.1	restriction endonuclease subunit S	-
LGL88_RS15065 (LGL88_15100)	3276482..3276772	-	291	WP_038275268.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LGL88_RS15070 (LGL88_15105)	3276762..3277016	-	255	WP_005175264.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
LGL88_RS15080 (LGL88_15115)	3278692..3279114	-	423	WP_230675767.1	very short patch repair endonuclease	-
LGL88_RS15085 (LGL88_15120)	3279365..3280801	+	1437	WP_049689835.1	DNA cytosine methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3273407..3287015	13608
	-	inside	IScluster/Tn	-	-	3272408..3278346	5938

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-75)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11494.42 Da
• Isoelectric Point: 9.9816

>T218959 WP_038275268.1 NZ_CP084641:c3276772-3276482 [Yersinia ruckeri]
MTFNIDFDERALKEWHKLDKTIREQFKKKLRKLQENPYIESARLHGDLAGCFKIKLRASGFRLIYQVIDEEIVIWIVAVG
KREDEKAYDIAKKRIQ

Nucleotide

• Length: 291 bp

>T218959 NZ_CP116145:c758990-758736 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9680.07 Da
• Isoelectric Point: 4.8283

>AT218959 WP_005175264.1 NZ_CP084641:c3277016-3276762 [Yersinia ruckeri]
MSTIQIRVDDELKKSAYQAFEKLNLSPSDALRLFLRYVAENEKLPFSEVSVMVSNQDEDADILAVVRERLKNPAKRIRVN
IDDL

Nucleotide

• Length: 255 bp

>AT218959 NZ_CP116145:c759238-758987 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A0T9LYG3

Antitoxin

• AlphaFold DB: A1JJ37