Detailed information of TA system TA218921

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 1954664..1955582
• Replicon: chromosome
• Accession: NZ_CP084637
• Organism: Yersinia ruckeri strain 17Y0412

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LGL85_RS08995
• Protein ID: WP_038242633.1
• Coordinates: 1955124..1955582 (+)
• Length: 153 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A085U7R3
• Locus tag: LGL85_RS08990
• Protein ID: WP_038242631.1
• Coordinates: 1954664..1955110 (+)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGL85_RS08970 (LGL85_08985)	1950362..1951066	+	705	WP_004717543.1	murein tripeptide amidase MpaA	-
LGL85_RS08975 (LGL85_08990)	1951077..1952051	-	975	WP_071706618.1	L-Ala-D/L-Glu epimerase	-
LGL85_RS08980 (LGL85_08995)	1952201..1952704	+	504	WP_004717547.1	thiol peroxidase	-
LGL85_RS08985 (LGL85_09000)	1952739..1954346	-	1608	WP_096823414.1	transcriptional regulator TyrR	-
LGL85_RS08990 (LGL85_09005)	1954664..1955110	+	447	WP_038242631.1	DUF2384 domain-containing protein	Antitoxin
LGL85_RS08995 (LGL85_09010)	1955124..1955582	+	459	WP_038242633.1	RES domain-containing protein	Toxin
LGL85_RS09000 (LGL85_09015)	1955586..1956644	-	1059	WP_038242635.1	YcjF family protein	-
LGL85_RS09005 (LGL85_09020)	1956641..1958038	-	1398	WP_004717556.1	YcjX family protein	-
LGL85_RS09010 (LGL85_09025)	1958019..1958261	-	243	WP_096823415.1	phage shock protein PspD	-
LGL85_RS09015 (LGL85_09030)	1958325..1958684	-	360	WP_004717559.1	envelope stress response membrane protein PspC	-
LGL85_RS09020 (LGL85_09035)	1958684..1958911	-	228	WP_038242637.1	envelope stress response membrane protein PspB	-
LGL85_RS09025 (LGL85_09040)	1958995..1959660	-	666	WP_038275968.1	phage shock protein PspA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-147)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (94-145)

Sequences

Toxin

Protein

• Length: 153 a.a.
• Molecular weight: 17072.55 Da
• Isoelectric Point: 6.6384

>T218921 WP_038242633.1 NZ_CP084637:1955124-1955582 [Yersinia ruckeri]
VMLYRIVHNRYLATAWTGYGAETYGGRWNHKGHAAIYLASSVSLAMLETLVHVEDSATLNDFNLFQITIPDNAIMVLQQQ
DWPRGWRDDPTPVATMDIGTEWIGSVSSVGLLVPSTLVPLERNMLVNPKHRDFASCLKSINPLTFSFDPRLK

Nucleotide

• Length: 459 bp

>T218921 NZ_CP116124:c82591-82286 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16406.87 Da
• Isoelectric Point: 8.0956

>AT218921 WP_038242631.1 NZ_CP084637:1954664-1955110 [Yersinia ruckeri]
MRTYHPSPMAKVSALWREIGLPASRGTALVDSIKLGFTVDVIDTIHLWADMPKSEILRATGIPSRSLTRRRTHDGRFTPE
ESERIARFVRVMDAAVDLFGGDRTKATAWMSMPVKGLGNRTPDSLLETETGALEICDLIGRLEHGVFS

Nucleotide

• Length: 447 bp

>AT218921 NZ_CP116124:c82811-82593 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A085U7R3