Detailed information of TA system TA021892

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3063615..3064185
• Replicon: chromosome
• Accession: NC_008769
• Organism: Mycobacterium bovis BCG str. Pasteur 1173P2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P71650
• Locus tag: BCG_RS14515
• Protein ID: WP_003414166.1
• Coordinates: 3063615..3063971 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P0CL61
• Locus tag: BCG_RS14520
• Protein ID: WP_003901465.1
• Coordinates: 3063955..3064185 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCG_RS14495	3059067..3060755	-	1689	WP_003414155.1	alpha/beta hydrolase family protein	-
BCG_RS14500	3060759..3061085	-	327	WP_003414157.1	hypothetical protein	-
BCG_RS14505	3061258..3061845	+	588	WP_003914429.1	DUF3558 family protein	-
BCG_RS14510	3061864..3063513	+	1650	WP_003899485.1	CocE/NonD family hydrolase	-
BCG_RS14515	3063615..3063971	-	357	WP_003414166.1	type II toxin-antitoxin system toxin endoribonuclease MazF9	Toxin
BCG_RS14520	3063955..3064185	-	231	WP_003901465.1	antitoxin MazE	Antitoxin
BCG_RS14525	3064228..3065271	-	1044	WP_003414172.1	DUF2293 domain-containing protein	-
BCG_RS22020	3065462..3065737	+	276	WP_003911993.1	DUF1778 domain-containing protein	-
BCG_RS22025	3065913..3066167	-	255	WP_003917684.1	hypothetical protein	-
BCG_RS14540	3066315..3066719	+	405	WP_003414181.1	hypothetical protein	-
BCG_RS14545	3066716..3066907	+	192	WP_003414184.1	hypothetical protein	-
BCG_RS14550	3066971..3068260	+	1290	Protein_2840	transposase family protein	-
BCG_RS14555	3068494..3068751	+	258	WP_003899489.1	hypothetical protein	-
BCG_RS14560	3068856..3069167	+	312	WP_010950777.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-29)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12858.73 Da
• Isoelectric Point: 9.1962

>T21892 WP_003414166.1 NC_008769:c3063971-3063615 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Nucleotide

• Length: 357 bp

>T21892 NC_008769:c3063971-3063615 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8231.01 Da
• Isoelectric Point: 4.1809

>AT21892 WP_003901465.1 NC_008769:c3064185-3063955 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Nucleotide

• Length: 231 bp

>AT21892 NC_008769:c3064185-3063955 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Structures

Toxin

• PDB: 6L2A
• PDB: 5HJZ
• PDB: 6KYT
• PDB: 6L29
• PDB: 7DU5
• PDB: 6KYS

Antitoxin

• AlphaFold DB: A0A7U4FBW3