Detailed information of TA system TA218904

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 26271..26755
• Replicon: plasmid unnamed2
• Accession: NZ_CP084594
• Organism: Mycobacterium intracellulare subsp. chimaera strain 850

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: LFT51_RS29015
• Protein ID: WP_046182256.1
• Coordinates: 26435..26755 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: LFT51_RS29010
• Protein ID: WP_232010004.1
• Coordinates: 26271..26438 (+)
• Length: 56 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LFT51_RS28990 (LFT51_28990)	22603..22809	-	207	WP_046182259.1	hypothetical protein	-
LFT51_RS28995 (LFT51_28995)	23306..23815	+	510	WP_046182258.1	hypothetical protein	-
LFT51_RS29000 (LFT51_29000)	24172..25311	-	1140	WP_046182268.1	hypothetical protein	-
LFT51_RS29005 (LFT51_29005)	25543..26124	-	582	WP_046182267.1	hypothetical protein	-
LFT51_RS29010 (LFT51_29010)	26271..26438	+	168	WP_232010004.1	antitoxin MazE family protein	Antitoxin
LFT51_RS29015 (LFT51_29015)	26435..26755	+	321	WP_046182256.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LFT51_RS29020 (LFT51_29020)	26838..28679	-	1842	WP_046182255.1	fatty acyl-AMP ligase	-
LFT51_RS29025 (LFT51_29025)	28964..29242	-	279	WP_046182254.1	hypothetical protein	-
LFT51_RS29030 (LFT51_29030)	29845..30204	-	360	WP_052742707.1	putative toxin-antitoxin system toxin component, PIN family	-
LFT51_RS29035 (LFT51_29035)	30259..30519	-	261	WP_046182265.1	hypothetical protein	-
LFT51_RS29040 (LFT51_29040)	30674..31225	+	552	WP_232010002.1	ParA family protein	-
LFT51_RS29045 (LFT51_29045)	31222..31671	+	450	WP_046182252.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..39887	39887

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-101)

Antitoxin

• Antitoxin MazE-like (1-52)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 11211.10 Da
• Isoelectric Point: 10.8056

>T218904 WP_046182256.1 NZ_CP084594:26435-26755 [Mycobacterium intracellulare subsp. chimaera]
VNRGELWTVSGGVYAAKPRPALIIQDNLFSATESVVVIPLTTTIADAPIARIAVPTTTGIAQPSFAMIDKITTVRRSNLG
TRVGRVSATLMADIERSLMVFLGLAS

Nucleotide

• Length: 321 bp

>T218904 NZ_CP116123:2202803-2202906 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 56 a.a.
• Molecular weight: 6313.95 Da
• Isoelectric Point: 4.3696

>AT218904 WP_232010004.1 NZ_CP084594:26271-26438 [Mycobacterium intracellulare subsp. chimaera]
MRERGYRPVQVWVPDVRSARFAAEAHREALALAEADRHSDDMDFVEAISDLVDNE

Nucleotide

• Length: 168 bp

>AT218904 NZ_CP116123:c2202944-2202673 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGCCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT