Detailed information of TA system TA218900

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YoeB-YefM
• Location: 54989..55527
• Replicon: plasmid unnamed1
• Accession: NZ_CP084593
• Organism: Mycobacterium intracellulare subsp. chimaera strain 850

Toxin (Protein)

• Gene name: relE
• Uniprot ID: D5P5D7
• Locus tag: LFT51_RS28705
• Protein ID: WP_007172405.1
• Coordinates: 54989..55246 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: M1FTM7
• Locus tag: LFT51_RS28710
• Protein ID: WP_007172404.1
• Coordinates: 55243..55527 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LFT51_RS28680 (LFT51_28680)	50764..50976	-	213	WP_007172410.1	hypothetical protein	-
LFT51_RS28685 (LFT51_28685)	51095..52195	-	1101	WP_007172409.1	tyrosine-type recombinase/integrase	-
LFT51_RS28690 (LFT51_28690)	52185..52754	-	570	WP_007172408.1	hypothetical protein	-
LFT51_RS28695 (LFT51_28695)	53373..54464	+	1092	WP_007172407.1	hypothetical protein	-
LFT51_RS28700 (LFT51_28700)	54481..54774	+	294	WP_007172406.1	hypothetical protein	-
LFT51_RS28705 (LFT51_28705)	54989..55246	-	258	WP_007172405.1	Txe/YoeB family addiction module toxin	Toxin
LFT51_RS28710 (LFT51_28710)	55243..55527	-	285	WP_007172404.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LFT51_RS28715 (LFT51_28715)	55609..56370	-	762	WP_023363524.1	hypothetical protein	-
LFT51_RS28720 (LFT51_28720)	56373..57095	-	723	WP_139315444.1	ParA family protein	-
LFT51_RS28725 (LFT51_28725)	57792..58652	+	861	WP_023363522.1	DUF2637 domain-containing protein	-
LFT51_RS29550	58874..60394	+	1521	WP_007172379.1	DUF4226 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	eccA5 / esxN	1..97267	97267

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (4-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-70)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10156.56 Da
• Isoelectric Point: 9.8613

>T218900 WP_007172405.1 NZ_CP084593:c55246-54989 [Mycobacterium intracellulare subsp. chimaera]
VRSINFDPAAWEDFLFWLASDRKTARRITRLIAEIQRNPFAGIGKPEPLKGELSGYWSRRIDDEHRIVYRADDHEIKILK
ARYHY

Nucleotide

• Length: 258 bp

>T218900 NZ_CP116123:c860598-860221 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTGTC
CCGACTACTGGGCCAGCATTACGGCCTTACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCAGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10455.62 Da
• Isoelectric Point: 4.3885

>AT218900 WP_007172404.1 NZ_CP084593:c55527-55243 [Mycobacterium intracellulare subsp. chimaera]
MAISASEARQRLFPLLEQVNTDHEPVRITSKAGDAVLMSAADYDSWQETVYLLRSPENARRLMEAVARDNATRPTPGSYT
KSIDELEEMAGGQE

Nucleotide

• Length: 285 bp

>AT218900 NZ_CP116123:c861056-860688 [Escherichia coli]
ATGTCGGACACACTCCCCGGAACAACGCTTCCCGACAACAACAAAGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCGGATGCGAACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGTTCACCAGCAGT
GAACTGAATCCCCACCGTCAGAATACCGTCACGCTGTATGCTAAAGGGCTGACCTGCCATGCGGACACCCTCGGCAGCTG
TGGTTACGTTTATCTTGCTGTTTATCCGACGCCCGAAACGAAACAGTAA

Structures

Toxin

• AlphaFold DB: A0A1Y0THX2

Antitoxin

• AlphaFold DB: M1FTM7