Detailed information of TA system TA218899

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Rv0298-Rv0299/-
• Location: 4542518..4543044
• Replicon: chromosome
• Accession: NZ_CP084592
• Organism: Mycobacterium intracellulare subsp. chimaera strain 850

Toxin (Protein)

• Gene name: Rv0299
• Uniprot ID: S4ZDZ6
• Locus tag: LFT51_RS21055
• Protein ID: WP_008259048.1
• Coordinates: 4542742..4543044 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: Rv0298
• Uniprot ID: S4ZFD0
• Locus tag: LFT51_RS21050
• Protein ID: WP_008259047.1
• Coordinates: 4542518..4542745 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LFT51_RS21025 (LFT51_21025)	4537589..4538701	-	1113	WP_226057885.1	MinD/ParA family protein	-
LFT51_RS21030 (LFT51_21030)	4538760..4539791	-	1032	WP_042912751.1	ATP-dependent 6-phosphofructokinase	-
LFT51_RS21035 (LFT51_21035)	4539835..4541328	-	1494	WP_042912754.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA	-
LFT51_RS21040 (LFT51_21040)	4541325..4541624	-	300	WP_009952003.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC	-
LFT51_RS21045 (LFT51_21045)	4541724..4542380	+	657	WP_087139897.1	amino acid-binding protein	-
LFT51_RS21050 (LFT51_21050)	4542518..4542745	+	228	WP_008259047.1	ribbon-helix-helix protein, CopG family	Antitoxin
LFT51_RS21055 (LFT51_21055)	4542742..4543044	+	303	WP_008259048.1	hypothetical protein	Toxin
LFT51_RS21060 (LFT51_21060)	4543160..4543468	-	309	WP_008259049.1	hypothetical protein	-
LFT51_RS21065 (LFT51_21065)	4543527..4546448	-	2922	WP_014712101.1	MMPL family transporter	-
LFT51_RS21070 (LFT51_21070)	4546445..4546849	-	405	WP_008259052.1	MmpS family transport accessory protein	-
LFT51_RS21075 (LFT51_21075)	4547096..4547614	+	519	WP_042912757.1	pyridoxamine 5'-phosphate oxidase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Ribbon-helix-helix protein, CopG (6-41)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10473.31 Da
• Isoelectric Point: 4.5636

>T218899 WP_008259048.1 NZ_CP084592:4542742-4543044 [Mycobacterium intracellulare subsp. chimaera]
MIAPGDIAPRRDTRRDLYVAVLSNAIHLAANTGRVIVCPFIPGKIPDDTMAMVVPLTQPEGTLLPELVQWLPTSALGEPI
GAVSAAALSEAMSIVTALIT

Nucleotide

• Length: 303 bp

>T218899 NZ_CP116123:781617-781913 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8173.14 Da
• Isoelectric Point: 6.4608

>AT218899 WP_008259047.1 NZ_CP084592:4542518-4542745 [Mycobacterium intracellulare subsp. chimaera]
MAKEKISVTVDAAVLADADSDAKAAGLNRSEMIERALRNEHLRRALHTYTTRTAPALNIDSYAGQVYEANRAADL

Nucleotide

• Length: 228 bp

>AT218899 NZ_CP116123:781915-782310 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• AlphaFold DB: S4ZDZ6

Antitoxin

• AlphaFold DB: S4ZFD0