Detailed information of TA system TA218893

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1358010..1358663
• Replicon: chromosome
• Accession: NZ_CP084592
• Organism: Mycobacterium intracellulare subsp. chimaera strain 850

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: LFT51_RS06835
• Protein ID: WP_008253911.1
• Coordinates: 1358202..1358663 (+)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: X8A187
• Locus tag: LFT51_RS06830
• Protein ID: WP_009957321.1
• Coordinates: 1358010..1358198 (+)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LFT51_RS06820 (LFT51_06820)	1353983..1355560	+	1578	WP_042911067.1	serine hydrolase	-
LFT51_RS06825 (LFT51_06825)	1355557..1357944	+	2388	WP_042910814.1	cation-translocating P-type ATPase	-
LFT51_RS06830 (LFT51_06830)	1358010..1358198	+	189	WP_009957321.1	antitoxin	Antitoxin
LFT51_RS06835 (LFT51_06835)	1358202..1358663	+	462	WP_008253911.1	SRPBCC family protein	Toxin
LFT51_RS06840 (LFT51_06840)	1358750..1359520	+	771	WP_042910815.1	VOC family protein	-
LFT51_RS06845 (LFT51_06845)	1359594..1360028	+	435	WP_008253914.1	hypothetical protein	-
LFT51_RS06850 (LFT51_06850)	1360033..1360521	-	489	WP_036454690.1	glyoxalase/bleomycin resistance/dioxygenase family protein	-
LFT51_RS06855 (LFT51_06855)	1360521..1362035	-	1515	WP_042910816.1	carotenoid oxygenase family protein	-
LFT51_RS06860 (LFT51_06860)	1362038..1363258	-	1221	WP_042910817.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-52)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 16639.20 Da
• Isoelectric Point: 8.5617

>T218893 WP_008253911.1 NZ_CP084592:1358202-1358663 [Mycobacterium intracellulare subsp. chimaera]
MAKLSGSIDVPLPPEVAWQHASDLSRYKDWLTIHRVWRSALPDEIEKGSVVESIVEVKGMPNRIKWTVVRYKPPEGMTLN
GDGVGGVKVKLMAKIQPKGDGSLVSFDVHLGGPALFGPIGMIVAAALRGDIDQSLENFVTVFTRPDPSSNGHR

Nucleotide

• Length: 462 bp

>T218893 NZ_CP116123:c72957-72580 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTGTC
CCGGTTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCCCGCCGGGCGACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6698.30 Da
• Isoelectric Point: 4.4702

>AT218893 WP_009957321.1 NZ_CP084592:1358010-1358198 [Mycobacterium intracellulare subsp. chimaera]
MGFLDKAKDLLSQNADKVETAIDKAGEFVDEKTQGKYSDTIHQVQEQAKKAAGAADTGDQQN

Nucleotide

• Length: 189 bp

>AT218893 NZ_CP116123:c73414-73046 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Antitoxin

• AlphaFold DB: X8A187