Detailed information of TA system TA218888

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YoeB-YefM
• Location: 78268..78806
• Replicon: plasmid unnamed2
• Accession: NZ_CP084588
• Organism: Mycobacterium intracellulare subsp. chimaera strain 852

Toxin (Protein)

• Gene name: relE
• Uniprot ID: D5P5D7
• Locus tag: LFT50_RS30410
• Protein ID: WP_007172405.1
• Coordinates: 78549..78806 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: M1FTM7
• Locus tag: LFT50_RS30405
• Protein ID: WP_007172404.1
• Coordinates: 78268..78552 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LFT50_RS31040	73401..74921	-	1521	WP_007172379.1	DUF4226 domain-containing protein	-
LFT50_RS30390 (LFT50_30390)	75143..75898	-	756	WP_074021258.1	DUF2637 domain-containing protein	-
LFT50_RS30395 (LFT50_30395)	76700..77422	+	723	WP_139315444.1	ParA family protein	-
LFT50_RS30400 (LFT50_30400)	77425..78186	+	762	WP_023363524.1	hypothetical protein	-
LFT50_RS30405 (LFT50_30405)	78268..78552	+	285	WP_007172404.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LFT50_RS30410 (LFT50_30410)	78549..78806	+	258	WP_007172405.1	Txe/YoeB family addiction module toxin	Toxin
LFT50_RS30415 (LFT50_30415)	79021..79314	-	294	WP_007172406.1	hypothetical protein	-
LFT50_RS30420 (LFT50_30420)	79331..80302	-	972	WP_226057957.1	hypothetical protein	-
LFT50_RS30425 (LFT50_30425)	81041..81610	+	570	WP_007172408.1	hypothetical protein	-
LFT50_RS30430 (LFT50_30430)	81600..82700	+	1101	WP_007172409.1	tyrosine-type recombinase/integrase	-
LFT50_RS30435 (LFT50_30435)	82819..83031	+	213	WP_007172410.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	eccA5 / esxN	1..95688	95688

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (4-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-70)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10156.56 Da
• Isoelectric Point: 9.8613

>T218888 WP_007172405.1 NZ_CP084588:78549-78806 [Mycobacterium intracellulare subsp. chimaera]
VRSINFDPAAWEDFLFWLASDRKTARRITRLIAEIQRNPFAGIGKPEPLKGELSGYWSRRIDDEHRIVYRADDHEIKILK
ARYHY

Nucleotide

• Length: 258 bp

>T218888 NZ_CP116116:41339-41824 [Escherichia coli]
GTGGGATGTGTAACAGCACCAGAACCTTTGTCTGCTTTTCATCAGGTGGCTGAGTTCGTCAGCGGTGAGACTGTGCTCGA
TGACTGGTTAAAGCAGAAAGGCCTCAAAAACCAGGCTCTCGGAGCGGCCCGAACGTTTGTAGTGTGTAAGAAAGACACAA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGGAGCGTCAACCATACAGAAGCGACAGGCAATCTTCGGCGTAACATG
CCAAATCCCATCCCTGTCATTATACTAGCCCGTCTTGCAGTCGATCTCTCTTTTCGCGGAAAAGGGCTTGGCGCTGATTT
ACTTCATGATGCGGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATTATGGTTCACGCACTCACCG
AAGAAGCTAAAAATTTCTACATTCACCATGGTTTCAAACCATCACAAACTCAACAACGGACATTATTCCTTAAACTCCCT
CAATAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10455.62 Da
• Isoelectric Point: 4.3885

>AT218888 WP_007172404.1 NZ_CP084588:78268-78552 [Mycobacterium intracellulare subsp. chimaera]
MAISASEARQRLFPLLEQVNTDHEPVRITSKAGDAVLMSAADYDSWQETVYLLRSPENARRLMEAVARDNATRPTPGSYT
KSIDELEEMAGGQE

Nucleotide

• Length: 285 bp

>AT218888 NZ_CP116116:41064-41351 [Escherichia coli]
ATGCTATACAAGGAGTATCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCCAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAATGGCCTGTCAGGCTGCCGAGAATGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGCGGAATTCATCGATATGCTTGATGCACCCGTTACAGATGAT
CCCGTTATTGAGAAACTGCTAGCAAGGAAACCTCAGTGGGATGTGTAA

Structures

Toxin

• AlphaFold DB: A0A1Y0THX2

Antitoxin

• AlphaFold DB: M1FTM7